Diversity of Fusarium spp. associated with dry rot of potato tubers in Poland

• Published in: European journal of plant pathology 2016-08, Vol.145 (4), p.871-884
• Main Authors: Stefańczyk, Emil, Sobkowiak, Sylwester, Brylińska, Marta, Śliwka, Jadwiga
• Format: Article
• Language: English
• Subjects: Agriculture; Ascomycota; Biodiversity; Biomedical and Life Sciences; Ecology; Fusarium; Laboratory tests; Life Sciences; Mycotoxins; Pathogens; Plant diseases; Plant Pathology; Plant Sciences; Potatoes; Solanum tuberosum; Vegetables
• ISSN: 0929-1873; 1573-8469
• DOI: 10.1007/s10658-016-0875-0

Fusarium spp. belong to the division Ascomycota and cause important plant diseases; these fungi may contaminate food products with mycotoxins, endangering human and animal health. Several Fusarium spp. have been associated with potato dry rot. The most frequent and devastating of these species are F. sambucinum , F. solani and F. oxysporum , depending on the geographic location and the season. Samples of potato tubers with dry rot symptoms were collected, and their putative fungal isolates were identified as Fusarium species using partial nucleotide sequences of the internal transcribed spacer, translation elongation factor 1-α and β-tubulin genes. Among 149 isolates, 12 species were identified. F. oxysporum was the most frequent (45 % of the isolates), followed by F. avenaceum (12.1 %), F. solani (10.7 %) and F. sambucinum (7.4 %). Phylogenetic analyses confirmed the species identifications and revealed a high diversity of F. solani and a low diversity of F. oxysporum . Potential producers of zearalenone and trichothecenes were identified within the obtained isolates using PCR markers. Isolates that were pathogenic to potatoes in laboratory tests were found in four species: F. sambucinum , F. avenaceum , F. culmorum , and F. graminearum . The effects of increased temperature and mixed inoculum on the pathogenicities of chosen species were evaluated. This study adds 434 potato-derived Fusarium sequences to the NCBI GenBank database and demonstrates that the list of Fusarium species and mycotoxins present in potato tubers may be richer than previously believed, regardless of whether these species cause dry rot or live as saprophytes.