Simulation of the inhibition of microbial sulfate reduction in a two-compartment upflow bioreactor subjected to molybdate injection

• Published in: Bioprocess and biosystems engineering 2016-08, Vol.39 (8), p.1201-1211
• Main Authors: de Jesus, E. B., de Andrade Lima, L. R. P.
• Format: Article
• Language: English
• Subjects: Bacteria; Bioengineering; Bioreactors; Biotechnology; Chemistry; Chemistry and Materials Science; Environmental Engineering/Biotechnology; Fluid mechanics; Food Science; Hydrodynamics; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Injection; Injection wells; Membrane reactors; Microbiology; Models, Theoretical; Molybdenum - chemistry; Oil and gas fields; Oil recovery; Oil reservoirs; Original Paper; Oxidation-Reduction; Secondary oil recovery; Sulfate reduction; Sulfates; Sulfates - metabolism
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-016-1598-5

Souring of oil fields during secondary oil recovery by water injection occurs mainly due to the action of sulfate-reducing bacteria (SRB) adhered to the rock surface in the vicinity of injection wells. Upflow packed-bed bioreactors have been used in petroleum microbiology because of its similarity to the oil field near the injection wells or production. However, these reactors do not realistically describe the regions near the injection wells, which are characterized by the presence of a saturated zone and a void region close to the well. In this study, the hydrodynamics of the two-compartment packing-free/packed-bed pilot bioreactor that mimics an oil reservoir was studied. The packed-free compartment was modeled using a continuous stirred tank model with mass exchange between active and stagnant zones, whereas the packed-bed compartment was modeled using a piston-dispersion-exchange model. The proposed model adequately represents the hydrodynamic of the packed-free/packed-bed bioreactor while the simulations provide important information about the characteristics of the residence time distribution (RTD) curves for different sets of model parameters. Simulations were performed to represent the control of the sulfate-reducing bacteria activity in the bioreactor with the use of molybdate in different scenarios. The simulations show that increased amounts of molybdate cause an effective inhibition of the souring sulfate-reducing bacteria activity.