A search for microscopic fungi with directed hydroxylase activity for the synthesis of steroid drugs

The hydroxylase activities of new strains such as Curvularia lunata , C. geniculata , C. eragrostidis , C. prasadii , Ulocladium botrytis , Alternaria tenuis , and Fusarium oxysporum toward three steroid substrates, namely, androstenedione (AD), cortexolone (S), and dehydroepiandrosterone acetate (D...

Full description

Saved in:
Bibliographic Details
Published in:Applied biochemistry and microbiology 2016-05, Vol.52 (3), p.316-323
Main Authors: Karpova, N. V., Andryushina, V. A., Stytsenko, T. S., Druzhinina, A. V., Feofanova, T. D., Kurakov, A. V.
Format: Article
Language:English
Subjects:
Alternaria
Biochemistry
Biomedical and Life Sciences
Biosynthesis
Botrytis
Curvularia lunata
Enzymes
Fungi
Fusarium oxysporum
Life Sciences
Medical Microbiology
Microbiology
Steroids
Ulocladium
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The hydroxylase activities of new strains such as Curvularia lunata , C. geniculata , C. eragrostidis , C. prasadii , Ulocladium botrytis , Alternaria tenuis , and Fusarium oxysporum toward three steroid substrates, namely, androstenedione (AD), cortexolone (S), and dehydroepiandrosterone acetate (DAA), were characterized. The 9α-hydroxylase activity of C. lunata 1011 cells against S to form 9α-hydroxy-S was shown for the first time. It was found that C. geniculata 837 and F. oxysporum 11dn1 strains can hydroxylate substrates to form pharmacologically promising 7α-hydroxysteroids. C. geniculata 837 cells selectively hydroxylate AD, resulting in 7α-hydroxytestosterone, whereas F. oxysporum 11dn1 leads to the transformation of DAA to 7α-hydroxydehydroepiandrosterone.
ISSN:0003-6838
1608-3024
DOI:10.1134/S000368381603008X