Tailoring Novel PTFE Surface Properties: Promoting Cell Adhesion and Antifouling Properties via a Wet Chemical Approach

Many biomaterials used for tissue engineering applications lack cell-adhesiveness and, in addition, are prone to nonspecific adsorption of proteins. This is especially important for blood-contacting devices such as vascular grafts and valves where appropriate surface properties should inhibit the in...

Full description

Saved in:
Bibliographic Details
Published in:Bioconjugate chemistry 2016-05, Vol.27 (5), p.1216-1221
Main Authors: Gabriel, Matthias, Niederer, Kerstin, Becker, Marc, Raynaud, Christophe Michel, Vahl, Christian-Friedrich, Frey, Holger
Format: Article
Language:English
Subjects:
Bacterial Adhesion - drug effects
Biochemistry
Biofouling - prevention & control
Blood Platelets - cytology
Blood Platelets - drug effects
Cell Adhesion - drug effects
Cell growth
Human Umbilical Vein Endothelial Cells - cytology
Human Umbilical Vein Endothelial Cells - drug effects
Humans
Polymers
Polytetrafluoroethylene - chemistry
Polytetrafluoroethylene - pharmacology
Tissue engineering
Transplants & implants
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Many biomaterials used for tissue engineering applications lack cell-adhesiveness and, in addition, are prone to nonspecific adsorption of proteins. This is especially important for blood-contacting devices such as vascular grafts and valves where appropriate surface properties should inhibit the initial attachment of platelets and promote endothelial cell colonization. As a consequence, the long-term outcome of the implants would be improved and the need for anticoagulation therapy could be reduced or even abolished. Polytetrafluoroethylene (PTFE), a frequently used polymer for various medical applications, was wet-chemically activated and subsequently modified by grafting the endothelial cell (EC) specific peptide arginine-glutamic acid-aspartic acid-valine (REDV) using a bifunctional polyethylene glycol (PEG)-spacer (known to reduce platelet and nonspecific protein adhesion). Modified and control surfaces were both evaluated in terms of EC adhesion, colonization, and the attachment of platelets. In addition, samples underwent bacterial challenges. The results strongly suggested that PEG-mediated peptide immobilization renders PTFE an excellent substrate for cellular growth while simultaneously endowing the material with antifouling properties.
ISSN:1043-1802
1520-4812
DOI:10.1021/acs.bioconjchem.6b00047