Odontoblastic inductive potential of epithelial cells derived from human deciduous dental pulp

For the dentin regeneration, dental epithelial cells are indispensible and must possess odontoblastic induction capability. Epithelial cell-like stem cells were recently identified in human deciduous dental pulp (DPESCs). However, their cellular characteristics remain poorly defined. The purpose of...

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Bibliographic Details
Published in:Journal of molecular histology 2016-06, Vol.47 (3), p.345-351
Main Authors: Lee, Hye-Kyung, Park, Ji-Won, Seo, You-Mi, Kim, Ha Hoon, Lee, Gene, Bae, Hyun-Sook, Park, Joo-Cheol
Format: Article
Language:English
Subjects:
Biomarkers
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell Differentiation - genetics
Cells, Cultured
Dental Pulp - cytology
Developmental Biology
Epithelial Cells - cytology
Epithelial Cells - metabolism
Gene Expression
Humans
Life Sciences
Odontoblasts - cytology
Odontoblasts - metabolism
Original Paper
Promoter Regions, Genetic
Transcriptional Activation
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Summary:For the dentin regeneration, dental epithelial cells are indispensible and must possess odontoblastic induction capability. Epithelial cell-like stem cells were recently identified in human deciduous dental pulp (DPESCs). However, their cellular characteristics remain poorly defined. The purpose of this study was to characterize DPESCs compared to HAT-7 ameloblastic cells. Expression levels of ameloblast-specific markers [odontogenic ameloblast-associated protein (Odam), matrix metalloproteinase (Mmp)-20, amelogenin, and ameloblastin] were detected in DPESCs. Co-culturing odontoblastic MDPC-23 cells with DPESCs increased expression of odontoblast differentiation markers (Dmp1 and Dspp) from days 4 to 10, while the expression of bone sialoprotein rapidly decreased. MDPC-23 cells cultured in DPESC-conditioned medium (CM) showed increased Dspp promoter activity compared with control MDPC-23 cultures. Mineralization was first observed in the CM groups from day 4 and proceeded rapidly until day 14, whereas mineralized nodules were found from day 7 in control media-cultured cells. In conclusion, DPESCs in human deciduous pulp possess ameloblast-like characteristics and differentiation properties, and substances derived from DPESCs promote odontoblastic differentiation. Thus, our results indicate that DPESCs can be a realistic epithelial source for use in odontoblastic induction and dentin formation of dental mesenchymal cells.
ISSN:1567-2379
1567-2387
DOI:10.1007/s10735-016-9676-1