Loading…

F2 screen for resistance to Bacillus thuringiensis Cry2Ab2-maize in field populations of Spodoptera frugiperda (Lepidoptera: Noctuidae) from the southern United States

[Display omitted] •215 F2 two-parent families of Spodoptera frugiperda were established from four states of the southern U.S.•Neonates of each family were screened on Cry2Ab2 maize leaf tissue.•One family was identified to possess a major Cry2Ab2 resistance allele.•Six families were identified to ca...

Full description

Saved in:
Bibliographic Details
Published in:Journal of invertebrate pathology 2016-07, Vol.138, p.66-72
Main Authors: Niu, Ying, Qureshi, Jawwad A., Ni, Xinzhi, Head, Graham P., Price, Paula A., Meagher, Robert L., Kerns, David, Levy, Ronnie, Yang, Xiangbing, Huang, Fangneng
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c3445-1e85ff3e8f3e8407778b8a552285739b3152c4570757bb1db8634e8927201a053
cites cdi_FETCH-LOGICAL-c3445-1e85ff3e8f3e8407778b8a552285739b3152c4570757bb1db8634e8927201a053
container_end_page 72
container_issue
container_start_page 66
container_title Journal of invertebrate pathology
container_volume 138
creator Niu, Ying
Qureshi, Jawwad A.
Ni, Xinzhi
Head, Graham P.
Price, Paula A.
Meagher, Robert L.
Kerns, David
Levy, Ronnie
Yang, Xiangbing
Huang, Fangneng
description [Display omitted] •215 F2 two-parent families of Spodoptera frugiperda were established from four states of the southern U.S.•Neonates of each family were screened on Cry2Ab2 maize leaf tissue.•One family was identified to possess a major Cry2Ab2 resistance allele.•Six families were identified to carry minor Cry2Ab2 resistance alleles.•Major Cry2Ab2 resistance alleles in S. frugiperda were relatively rare in the southern U.S. The fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), is a target pest of transgenic maize and cotton expressing Bacillus thuringiensis (Bt) proteins in both North and South America. In 2013 and 2014, a total of 215 F2 two-parent families of S. frugiperda were established using single-pair mating of field individuals collected from seven locations in four states of the southern U.S.: Texas, Louisiana, Georgia, and Florida. The objective of the investigation was to detect resistance alleles in field populations to Cry2Ab2, a common Bt protein produced in transgenic maize and cotton. For each F2 family, 128 F2 neonates were screened on leaf tissue of Cry2Ab2 maize plants in the laboratory. A conservative estimate of the frequency of major Cry2Ab2 resistance alleles in S. frugiperda from the four states was 0.0023 with a 95% credibility interval of 0.0003–0.0064. In addition, six families were considered to likely possess minor resistance alleles at a frequency of 0.0082 with a 95% credibility interval of 0.0033–0.0152. One F2 family from Georgia (GA-15) was confirmed to possess a major resistance allele to the Cry2Ab2 protein. Larvae from this family survived well on whole maize plants expressing Cry2Ab2 protein and demonstrated a significant level (>15-fold) of resistance when fed with the same protein incorporated in a meridic diet. The detection of the major resistance allele along with the relatively abundant minor resistance alleles revealed in this study may have important implications for resistance management.
doi_str_mv 10.1016/j.jip.2016.06.005
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1808677830</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022201116300702</els_id><sourcerecordid>1808677830</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3445-1e85ff3e8f3e8407778b8a552285739b3152c4570757bb1db8634e8927201a053</originalsourceid><addsrcrecordid>eNqNkc9u1DAQxi1ERZfCA3BBPpZDlrETJw6cyqoFpFV7KD1bjjMpXiVx8B-k8kK8Zr3swhFVGmtGnt98tuYj5A2DNQNWv9-td3ZZ81yuIQeIZ2TFoK0LkCCekxUA50Vus1PyMoQd5ErU7QtyypuSMdnWK_L7itNgPOJMB-epx2BD1LNBGh39pI0dxxRo_J68ne8tzrlNN_6BX3S8mLT9hdTmSYtjTxe3pFFH6-ZA3UBvF9e7JaLXdPDp3i7oe03Pt7jY4_0Heu1MTLbX-C4zbsrvIA0u5eRnejfbiD29jTpieEVOBj0GfH3MZ-Tu6vLb5kuxvfn8dXOxLUxZVaJgKMUwlCj3p4KmaWQntRCcS9GUbVcywU0lGmhE03Ws72RdVihb3uQ1aRDlGTk_6C7e_UgYoppsMDiOekaXgmISZJ1VS3gKWnHgDGRG2QE13oXgcVCLt5P2D4qB2lupdipbqfZWKsjx5ydvj_Kpm7D_N_HXuwx8PACY9_HTolfBZIcM9tajiap39j_yj-3vr3I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1804202108</pqid></control><display><type>article</type><title>F2 screen for resistance to Bacillus thuringiensis Cry2Ab2-maize in field populations of Spodoptera frugiperda (Lepidoptera: Noctuidae) from the southern United States</title><source>ScienceDirect Journals</source><creator>Niu, Ying ; Qureshi, Jawwad A. ; Ni, Xinzhi ; Head, Graham P. ; Price, Paula A. ; Meagher, Robert L. ; Kerns, David ; Levy, Ronnie ; Yang, Xiangbing ; Huang, Fangneng</creator><creatorcontrib>Niu, Ying ; Qureshi, Jawwad A. ; Ni, Xinzhi ; Head, Graham P. ; Price, Paula A. ; Meagher, Robert L. ; Kerns, David ; Levy, Ronnie ; Yang, Xiangbing ; Huang, Fangneng</creatorcontrib><description>[Display omitted] •215 F2 two-parent families of Spodoptera frugiperda were established from four states of the southern U.S.•Neonates of each family were screened on Cry2Ab2 maize leaf tissue.•One family was identified to possess a major Cry2Ab2 resistance allele.•Six families were identified to carry minor Cry2Ab2 resistance alleles.•Major Cry2Ab2 resistance alleles in S. frugiperda were relatively rare in the southern U.S. The fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), is a target pest of transgenic maize and cotton expressing Bacillus thuringiensis (Bt) proteins in both North and South America. In 2013 and 2014, a total of 215 F2 two-parent families of S. frugiperda were established using single-pair mating of field individuals collected from seven locations in four states of the southern U.S.: Texas, Louisiana, Georgia, and Florida. The objective of the investigation was to detect resistance alleles in field populations to Cry2Ab2, a common Bt protein produced in transgenic maize and cotton. For each F2 family, 128 F2 neonates were screened on leaf tissue of Cry2Ab2 maize plants in the laboratory. A conservative estimate of the frequency of major Cry2Ab2 resistance alleles in S. frugiperda from the four states was 0.0023 with a 95% credibility interval of 0.0003–0.0064. In addition, six families were considered to likely possess minor resistance alleles at a frequency of 0.0082 with a 95% credibility interval of 0.0033–0.0152. One F2 family from Georgia (GA-15) was confirmed to possess a major resistance allele to the Cry2Ab2 protein. Larvae from this family survived well on whole maize plants expressing Cry2Ab2 protein and demonstrated a significant level (&gt;15-fold) of resistance when fed with the same protein incorporated in a meridic diet. The detection of the major resistance allele along with the relatively abundant minor resistance alleles revealed in this study may have important implications for resistance management.</description><identifier>ISSN: 0022-2011</identifier><identifier>EISSN: 1096-0805</identifier><identifier>DOI: 10.1016/j.jip.2016.06.005</identifier><identifier>PMID: 27311896</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Bacillus thuringiensis ; Bacterial Proteins - genetics ; Cry2Ab2 ; Endotoxins - genetics ; F2 screening ; Hemolysin Proteins - genetics ; Insecticide Resistance - genetics ; Lepidoptera ; Noctuidae ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - parasitology ; Resistance management ; Spodoptera - genetics ; Spodoptera frugiperda ; Transgenic maize ; United States ; Zea mays ; Zea mays - genetics ; Zea mays - parasitology</subject><ispartof>Journal of invertebrate pathology, 2016-07, Vol.138, p.66-72</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3445-1e85ff3e8f3e8407778b8a552285739b3152c4570757bb1db8634e8927201a053</citedby><cites>FETCH-LOGICAL-c3445-1e85ff3e8f3e8407778b8a552285739b3152c4570757bb1db8634e8927201a053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27311896$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Niu, Ying</creatorcontrib><creatorcontrib>Qureshi, Jawwad A.</creatorcontrib><creatorcontrib>Ni, Xinzhi</creatorcontrib><creatorcontrib>Head, Graham P.</creatorcontrib><creatorcontrib>Price, Paula A.</creatorcontrib><creatorcontrib>Meagher, Robert L.</creatorcontrib><creatorcontrib>Kerns, David</creatorcontrib><creatorcontrib>Levy, Ronnie</creatorcontrib><creatorcontrib>Yang, Xiangbing</creatorcontrib><creatorcontrib>Huang, Fangneng</creatorcontrib><title>F2 screen for resistance to Bacillus thuringiensis Cry2Ab2-maize in field populations of Spodoptera frugiperda (Lepidoptera: Noctuidae) from the southern United States</title><title>Journal of invertebrate pathology</title><addtitle>J Invertebr Pathol</addtitle><description>[Display omitted] •215 F2 two-parent families of Spodoptera frugiperda were established from four states of the southern U.S.•Neonates of each family were screened on Cry2Ab2 maize leaf tissue.•One family was identified to possess a major Cry2Ab2 resistance allele.•Six families were identified to carry minor Cry2Ab2 resistance alleles.•Major Cry2Ab2 resistance alleles in S. frugiperda were relatively rare in the southern U.S. The fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), is a target pest of transgenic maize and cotton expressing Bacillus thuringiensis (Bt) proteins in both North and South America. In 2013 and 2014, a total of 215 F2 two-parent families of S. frugiperda were established using single-pair mating of field individuals collected from seven locations in four states of the southern U.S.: Texas, Louisiana, Georgia, and Florida. The objective of the investigation was to detect resistance alleles in field populations to Cry2Ab2, a common Bt protein produced in transgenic maize and cotton. For each F2 family, 128 F2 neonates were screened on leaf tissue of Cry2Ab2 maize plants in the laboratory. A conservative estimate of the frequency of major Cry2Ab2 resistance alleles in S. frugiperda from the four states was 0.0023 with a 95% credibility interval of 0.0003–0.0064. In addition, six families were considered to likely possess minor resistance alleles at a frequency of 0.0082 with a 95% credibility interval of 0.0033–0.0152. One F2 family from Georgia (GA-15) was confirmed to possess a major resistance allele to the Cry2Ab2 protein. Larvae from this family survived well on whole maize plants expressing Cry2Ab2 protein and demonstrated a significant level (&gt;15-fold) of resistance when fed with the same protein incorporated in a meridic diet. The detection of the major resistance allele along with the relatively abundant minor resistance alleles revealed in this study may have important implications for resistance management.</description><subject>Animals</subject><subject>Bacillus thuringiensis</subject><subject>Bacterial Proteins - genetics</subject><subject>Cry2Ab2</subject><subject>Endotoxins - genetics</subject><subject>F2 screening</subject><subject>Hemolysin Proteins - genetics</subject><subject>Insecticide Resistance - genetics</subject><subject>Lepidoptera</subject><subject>Noctuidae</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - parasitology</subject><subject>Resistance management</subject><subject>Spodoptera - genetics</subject><subject>Spodoptera frugiperda</subject><subject>Transgenic maize</subject><subject>United States</subject><subject>Zea mays</subject><subject>Zea mays - genetics</subject><subject>Zea mays - parasitology</subject><issn>0022-2011</issn><issn>1096-0805</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNkc9u1DAQxi1ERZfCA3BBPpZDlrETJw6cyqoFpFV7KD1bjjMpXiVx8B-k8kK8Zr3swhFVGmtGnt98tuYj5A2DNQNWv9-td3ZZ81yuIQeIZ2TFoK0LkCCekxUA50Vus1PyMoQd5ErU7QtyypuSMdnWK_L7itNgPOJMB-epx2BD1LNBGh39pI0dxxRo_J68ne8tzrlNN_6BX3S8mLT9hdTmSYtjTxe3pFFH6-ZA3UBvF9e7JaLXdPDp3i7oe03Pt7jY4_0Heu1MTLbX-C4zbsrvIA0u5eRnejfbiD29jTpieEVOBj0GfH3MZ-Tu6vLb5kuxvfn8dXOxLUxZVaJgKMUwlCj3p4KmaWQntRCcS9GUbVcywU0lGmhE03Ws72RdVihb3uQ1aRDlGTk_6C7e_UgYoppsMDiOekaXgmISZJ1VS3gKWnHgDGRG2QE13oXgcVCLt5P2D4qB2lupdipbqfZWKsjx5ydvj_Kpm7D_N_HXuwx8PACY9_HTolfBZIcM9tajiap39j_yj-3vr3I</recordid><startdate>201607</startdate><enddate>201607</enddate><creator>Niu, Ying</creator><creator>Qureshi, Jawwad A.</creator><creator>Ni, Xinzhi</creator><creator>Head, Graham P.</creator><creator>Price, Paula A.</creator><creator>Meagher, Robert L.</creator><creator>Kerns, David</creator><creator>Levy, Ronnie</creator><creator>Yang, Xiangbing</creator><creator>Huang, Fangneng</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SS</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>201607</creationdate><title>F2 screen for resistance to Bacillus thuringiensis Cry2Ab2-maize in field populations of Spodoptera frugiperda (Lepidoptera: Noctuidae) from the southern United States</title><author>Niu, Ying ; Qureshi, Jawwad A. ; Ni, Xinzhi ; Head, Graham P. ; Price, Paula A. ; Meagher, Robert L. ; Kerns, David ; Levy, Ronnie ; Yang, Xiangbing ; Huang, Fangneng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3445-1e85ff3e8f3e8407778b8a552285739b3152c4570757bb1db8634e8927201a053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Bacillus thuringiensis</topic><topic>Bacterial Proteins - genetics</topic><topic>Cry2Ab2</topic><topic>Endotoxins - genetics</topic><topic>F2 screening</topic><topic>Hemolysin Proteins - genetics</topic><topic>Insecticide Resistance - genetics</topic><topic>Lepidoptera</topic><topic>Noctuidae</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - parasitology</topic><topic>Resistance management</topic><topic>Spodoptera - genetics</topic><topic>Spodoptera frugiperda</topic><topic>Transgenic maize</topic><topic>United States</topic><topic>Zea mays</topic><topic>Zea mays - genetics</topic><topic>Zea mays - parasitology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Niu, Ying</creatorcontrib><creatorcontrib>Qureshi, Jawwad A.</creatorcontrib><creatorcontrib>Ni, Xinzhi</creatorcontrib><creatorcontrib>Head, Graham P.</creatorcontrib><creatorcontrib>Price, Paula A.</creatorcontrib><creatorcontrib>Meagher, Robert L.</creatorcontrib><creatorcontrib>Kerns, David</creatorcontrib><creatorcontrib>Levy, Ronnie</creatorcontrib><creatorcontrib>Yang, Xiangbing</creatorcontrib><creatorcontrib>Huang, Fangneng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><jtitle>Journal of invertebrate pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Niu, Ying</au><au>Qureshi, Jawwad A.</au><au>Ni, Xinzhi</au><au>Head, Graham P.</au><au>Price, Paula A.</au><au>Meagher, Robert L.</au><au>Kerns, David</au><au>Levy, Ronnie</au><au>Yang, Xiangbing</au><au>Huang, Fangneng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>F2 screen for resistance to Bacillus thuringiensis Cry2Ab2-maize in field populations of Spodoptera frugiperda (Lepidoptera: Noctuidae) from the southern United States</atitle><jtitle>Journal of invertebrate pathology</jtitle><addtitle>J Invertebr Pathol</addtitle><date>2016-07</date><risdate>2016</risdate><volume>138</volume><spage>66</spage><epage>72</epage><pages>66-72</pages><issn>0022-2011</issn><eissn>1096-0805</eissn><abstract>[Display omitted] •215 F2 two-parent families of Spodoptera frugiperda were established from four states of the southern U.S.•Neonates of each family were screened on Cry2Ab2 maize leaf tissue.•One family was identified to possess a major Cry2Ab2 resistance allele.•Six families were identified to carry minor Cry2Ab2 resistance alleles.•Major Cry2Ab2 resistance alleles in S. frugiperda were relatively rare in the southern U.S. The fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), is a target pest of transgenic maize and cotton expressing Bacillus thuringiensis (Bt) proteins in both North and South America. In 2013 and 2014, a total of 215 F2 two-parent families of S. frugiperda were established using single-pair mating of field individuals collected from seven locations in four states of the southern U.S.: Texas, Louisiana, Georgia, and Florida. The objective of the investigation was to detect resistance alleles in field populations to Cry2Ab2, a common Bt protein produced in transgenic maize and cotton. For each F2 family, 128 F2 neonates were screened on leaf tissue of Cry2Ab2 maize plants in the laboratory. A conservative estimate of the frequency of major Cry2Ab2 resistance alleles in S. frugiperda from the four states was 0.0023 with a 95% credibility interval of 0.0003–0.0064. In addition, six families were considered to likely possess minor resistance alleles at a frequency of 0.0082 with a 95% credibility interval of 0.0033–0.0152. One F2 family from Georgia (GA-15) was confirmed to possess a major resistance allele to the Cry2Ab2 protein. Larvae from this family survived well on whole maize plants expressing Cry2Ab2 protein and demonstrated a significant level (&gt;15-fold) of resistance when fed with the same protein incorporated in a meridic diet. The detection of the major resistance allele along with the relatively abundant minor resistance alleles revealed in this study may have important implications for resistance management.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27311896</pmid><doi>10.1016/j.jip.2016.06.005</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0022-2011
ispartof Journal of invertebrate pathology, 2016-07, Vol.138, p.66-72
issn 0022-2011
1096-0805
language eng
recordid cdi_proquest_miscellaneous_1808677830
source ScienceDirect Journals
subjects Animals
Bacillus thuringiensis
Bacterial Proteins - genetics
Cry2Ab2
Endotoxins - genetics
F2 screening
Hemolysin Proteins - genetics
Insecticide Resistance - genetics
Lepidoptera
Noctuidae
Plants, Genetically Modified - genetics
Plants, Genetically Modified - parasitology
Resistance management
Spodoptera - genetics
Spodoptera frugiperda
Transgenic maize
United States
Zea mays
Zea mays - genetics
Zea mays - parasitology
title F2 screen for resistance to Bacillus thuringiensis Cry2Ab2-maize in field populations of Spodoptera frugiperda (Lepidoptera: Noctuidae) from the southern United States
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T08%3A21%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=F2%20screen%20for%20resistance%20to%20Bacillus%20thuringiensis%20Cry2Ab2-maize%20in%20field%20populations%20of%20Spodoptera%20frugiperda%20(Lepidoptera:%20Noctuidae)%20from%20the%20southern%20United%20States&rft.jtitle=Journal%20of%20invertebrate%20pathology&rft.au=Niu,%20Ying&rft.date=2016-07&rft.volume=138&rft.spage=66&rft.epage=72&rft.pages=66-72&rft.issn=0022-2011&rft.eissn=1096-0805&rft_id=info:doi/10.1016/j.jip.2016.06.005&rft_dat=%3Cproquest_cross%3E1808677830%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c3445-1e85ff3e8f3e8407778b8a552285739b3152c4570757bb1db8634e8927201a053%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1804202108&rft_id=info:pmid/27311896&rfr_iscdi=true