Effects of dietary tannic acid on the growth, hepatic gene expression, and antioxidant enzyme activity in Brandt's voles (Microtus brandti)

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2016-06, Vol.196-197, p.19-26
• Main Authors: Ye, Man-Hong, Nan, Yan-Lei, Ding, Meng-Meng, Hu, Jun-Bang, Liu, Qian, Wei, Wan-Hong, Yang, Sheng-Mei
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant enzymes; Antioxidants - metabolism; Arvicolinae - genetics; Arvicolinae - growth & development; Arvicolinae - metabolism; Body Weight - drug effects; Diet; Eating - drug effects; Food intake; Gene Amplification - drug effects; Gene expression; Gene Expression Regulation - drug effects; Hydroxymethylglutaryl CoA Reductases - genetics; Liver - drug effects; Liver - enzymology; Liver - growth & development; Liver - metabolism; Microtus; Organ Size - drug effects; Tannic acid; Tannins - pharmacology; Transferrin - genetics
• ISSN: 1096-4959; 1879-1107
• DOI: 10.1016/j.cbpb.2016.01.011

This study was designed to investigate the physiological and biochemical responses of Brandt's voles to the persistent presence of dietary tannic acid. The diet for animals in the experimental group was supplemented with 3% dietary tannic acid for 5weeks. The control group received a commercial lab chow. No significant differences were detected in body weight, organ (heart, kidney, and liver) weights, and organ parameters between animals from two groups. However, voles in the experimental group had significantly higher daily food intake, increased contents of proline and histidine in saliva and feces after protein hydrolysis, and elevated hepatic expression of transferrin than the control. Our results suggested the existence of adaptive strategies developed in Brandt's voles to overcome the adverse effects of dietary tannic acid. (1) Food consumption was increased to satisfy their nutritional demands. (2) The secretion of tannic-acid-binding salivary proteins was promoted. (3) The absorption of iron was enhanced. These alterations contributed to neutralize the negative effects of tannic acid and maintain body mass in animals supplemented with tannic acid. As the result of the consumption of tannic acid, hepatic expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase was significantly decreased, while the overall potential of the antioxidant system, characterized by increased hepatic enzymatic activities of catalase and glutathione peroxidase, was enhanced. Our results also implied the involvement of tannic acid in the regulation of lipid metabolism and oxidative stress in voles.