Synthesis and study on activity in vitro of the high purity human butyrylcholinesterase conjugated with gold nanoparticles

The aim of this research was to design a method of immobilization of high-purity human butyrylcholinesterase on the surface of gold nanoparticles preserving the activity of the enzyme. In order to achieve this aim, the method of fractionation and purification of human butyrylcholinesterase from plas...

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Published in:Doklady. Biochemistry and biophysics 2016-05, Vol.468 (1), p.232-234
Main Authors: Sokolov, O. I., Selivanov, N. Y., Bogatyrev, V. A., Selivanova, O. G., Velikorodnaya, Y. I., Pocheptsov, A. Y., Filatov, B. N., Shchyogolev, S. Y., Dykman, L. A.
Format: Article
Language:English
Subjects:
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Biophysics and Molecular Biology
Butyrylcholinesterase - chemistry
Butyrylcholinesterase - isolation & purification
Chemical synthesis
Enzyme Stability
Enzymes
Gold
Gold Compounds - chemical synthesis
Gold Compounds - chemistry
Humans
Hydrogen-Ion Concentration
Life Sciences
Metal Nanoparticles - chemistry
Nanoparticles
Particle Size
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Summary:The aim of this research was to design a method of immobilization of high-purity human butyrylcholinesterase on the surface of gold nanoparticles preserving the activity of the enzyme. In order to achieve this aim, the method of fractionation and purification of human butyrylcholinesterase from plasma was modified. The synthesis of 15-nm gold nanoparticles was carried out by citrated method. A method of conjugation of the high-purity butyrylcholinesterase with gold nanoparticles was developed. It was found that the Immobilization of butyrylcholinesterase on the surface of gold nanoparticles resulted in a significant (to 23%) increase in the specific activity of the enzyme.
ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672916030212