In vivo photodynamic inactivation of Candida albicans using chloro-aluminum phthalocyanine

This study evaluated the photoinactivation of Candida albicans in a murine model of oral candidiasis using chloro‐aluminum phthalocyanine (ClAlP) encapsulated in cationic nanoemulsions (NE) and chloro‐aluminum phthalocyanine (ClAlP) diluted in DMSO (DMSO) as photosensitizer (PS). Seventy‐five 6‐week...

Full description

Saved in:
Bibliographic Details
Published in:Oral diseases 2016-07, Vol.22 (5), p.415-422
Main Authors: Carmello, JC, Alves, F, Ribeiro, APD, Basso, FG, de Souza Costa, CA, Tedesco, AC, Primo, FL, Mima, EG, Pavarina, AC
Format: Article
Language:English
Subjects:
Animals
Candida albicans
Candida albicans - drug effects
Candida albicans - isolation & purification
Candida albicans - radiation effects
Candidiasis, Oral - drug therapy
Candidiasis, Oral - microbiology
chloro-aluminum phthalocyanine
Cytokines - analysis
Cytokines - genetics
Dentistry
Disease Models, Animal
Female
Fungal infections
Indoles - pharmacology
Mice
murine model
Oral diseases
Organometallic Compounds - pharmacology
Photochemotherapy - methods
Photosensitizing Agents - pharmacology
Random Allocation
Rodents
Tetracycline - pharmacology
Tongue - drug effects
Tongue - microbiology
Tongue - radiation effects
Yeast
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study evaluated the photoinactivation of Candida albicans in a murine model of oral candidiasis using chloro‐aluminum phthalocyanine (ClAlP) encapsulated in cationic nanoemulsions (NE) and chloro‐aluminum phthalocyanine (ClAlP) diluted in DMSO (DMSO) as photosensitizer (PS). Seventy‐five 6‐week‐old female Swiss mice were immunosuppressed and inoculated with C. albicans to induce oral candidiasis. PDT was performed on the tongue by the application of the photosensitizers and LED light (100 J cm−2–660 nm). Twenty‐four hours and 7 days after treatments, microbiological evaluation was carried out by recovering C. albicans from the tongue of animals (CFU ml−1). Then, mice were sacrificed and the tongues were surgically removed for histological and biomolecular analysis of pro‐ and anti‐inflammatory cytokines. Data were analyzed by ANOVA followed by Tukey's post hoc test. ClAlP‐NE‐mediated PDT reduced 2.26 log10 of C. albicans recovered from the tongue when compared with the control group (P−L−) (P 
ISSN:1354-523X
1601-0825
DOI:10.1111/odi.12466