Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japanese eel Anguilla japonica

Highlights • have obtained specific monoclonal antibodies against eel α-subunit and FSH β-subunit. • produced recombinant rec-tethered eel FSH and LH in the CHO cells. • established ELISA analysis method for rec-tethered eel FSH and LH.

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Bibliographic Details
Published in:General and comparative endocrinology 2016-07, Vol.233, p.8-15
Main Authors: Kim, Dae-Jung, Park, Chae-Won, Kim, Dong-Wan, Park, Hong-Kyu, Byambaragchaa, Munkhzaya, Lee, Nam-Sil, Hong, Sun-Mee, Seo, Mi-Young, Kang, Myung-Hwa, Min, Kwan-Sik
Format: Article
Language:English
Subjects:
Anguilla - immunology
Anguilla - metabolism
Anguilla japonica
Animals
Antibodies, Monoclonal - analysis
Antibodies, Monoclonal - immunology
Antibodies, Monoclonal - metabolism
CHO Cells
Cricetinae
Cricetulus
Endocrinology & Metabolism
Escherichia coli
Female
Follicle Stimulating Hormone - immunology
Follicle Stimulating Hormone - metabolism
Follicle Stimulating Hormone, beta Subunit - metabolism
Follicle-stimulating hormone
Japanese eel
Luteinizing Hormone, beta Subunit - metabolism
Marine
Monoclonal antibody
Oogenesis
Pituitary Gland - metabolism
Protein Binding
Recombinant
Recombinant Proteins - immunology
Recombinant Proteins - metabolism
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Description
Summary:Highlights • have obtained specific monoclonal antibodies against eel α-subunit and FSH β-subunit. • produced recombinant rec-tethered eel FSH and LH in the CHO cells. • established ELISA analysis method for rec-tethered eel FSH and LH.
ISSN:0016-6480
1095-6840
DOI:10.1016/j.ygcen.2016.04.030