NI-35 ESTABLISHMENT OF BRAIN TUMOR PHOSPHOLIPID IMAGING PROFILING BY MALDI MASS SPECTROMETRY - A MOUSE MODEL

• Published in: Neuro-oncology (Charlottesville, Va.) Va.), 2014-11, Vol.16 (suppl 5), p.v145-v145
• Main Authors: Huang, C.-Y., Wu, H.-W., Wang, H.-Y. J., Chen, P.-Y., Wei, K.-C.
• Format: Article
• Language: English
• ISSN: 1522-8517; 1523-5866
• DOI: 10.1093/neuonc/nou264.33

Malignant brain tumor is a common and severe primary brain tumor with a high recurrence rate and a poor prognosis. Because of the infiltrative nature of malignant brain tumor, tissues surrounding the tumor that remain after surgery might contribute to recurrence. Develop potential tumor marker profiles may help to distinguish tumor and healthy tissues in the brain. Since aberrant lipid metabolism in tumor cells has been commonly observed in cancer cells, it is likely that the altered lipid metabolism will also be seen in the in situ tumors. To visualize such change, we profiled the phospholipids in the brain tumor created in a mouse model with MALDI-MS. We also visualized the distribution of aberrant phospholipids in the brain tumor by MALDI-MS imaging. The MALDI profiling and imaging were also carried out in sections after desalting to remove the interference from the endogenous alkali metal ions, and reveal additional low abundant lysophospholipids within the tumor. Tumor specific phosphatidylcholine, lysophosphatidylcholine and sphingomyelin distribution also show regional variation within the brain tumor region. Whether the combination of these species can serve as the marker for pathological differential grading requires further verification.