Post-transcriptional gene silencing activity of human GIGYF2

In mammalian post-transcriptional gene silencing, the Argonaute protein AGO2 indirectly recruits translation inhibitors, deadenylase complexes, and decapping factors to microRNA-targeted mRNAs, thereby repressing mRNA translation and accelerating mRNA decay. However, the exact composition and assemb...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2016-07, Vol.475 (3), p.289-294
Main Authors: Kryszke, Marie-Hélène, Adjeriou, Badia, Liang, Feifei, Chen, Hong, Dautry, François
Format: Article
Language:English
Subjects:
Argonaute 2
Argonaute Proteins - metabolism
Carrier Proteins - metabolism
Gene Silencing
Genes, Reporter
GIGYF2
HEK293 Cells
HeLa Cells
Humans
Post-transcriptional gene silencing
Protein Biosynthesis
Protein Interaction Maps
RNA Stability
RNA, Messenger - chemistry
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA-induced silencing complex
TNRC6
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Summary:In mammalian post-transcriptional gene silencing, the Argonaute protein AGO2 indirectly recruits translation inhibitors, deadenylase complexes, and decapping factors to microRNA-targeted mRNAs, thereby repressing mRNA translation and accelerating mRNA decay. However, the exact composition and assembly pathway of the microRNA-induced silencing complex are not completely elucidated. As the GYF domain of human GIGYF2 was shown to bind AGO2 in pulldown experiments, we wondered whether GIGYF2 could be a novel protein component of the microRNA-induced silencing complex. Here we show that full-length GIGYF2 coimmunoprecipitates with AGO2 in human cells, and demonstrate that, upon tethering to a reporter mRNA, GIGYF2 exhibits strong, dose-dependent silencing activity, involving both mRNA destabilization and translational repression. •Human GIGYF2 may be a novel component of the microRNA-induced silencing complex.•When tethered to a reporter mRNA, GIGYF2 exerts a dose-dependent silencing activity.•Translational repression and mRNA decay contribute to GIGYF2 silencing activity.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2016.05.022