Growth inhibition and oxidative damage of Microcystis aeruginosa induced by crude extract of Sagittaria trifolia tubers

• Published in: Journal of environmental sciences (China) 2016-05, Vol.43 (5), p.40-47
• Main Authors: Li, Jiang, Liu, Yunguo, Zhang, Pingyang, Zeng, Guangming, Cai, Xiaoxi, Liu, Shaobo, Yin, Yicheng, Hu, Xinjiang, Hu, Xi, Tan, Xiaofei
• Format: Article
• Language: English
• Subjects: Antioxidant response; Chlorophyll; Cyanobacteria; Cyanobacterial inhibition; Glutathione - metabolism; Harmful Algal Bloom - drug effects; Lipid Peroxidation; Malondialdehyde - metabolism; Microcystis - drug effects; Microcystis aeruginosa; Oxidative damage; Oxidative Stress; Oxidoreductases - metabolism; Plant Extracts - toxicity; Reactive Oxygen Species - metabolism; Sagittaria; Sagittaria trifolia; Superoxide Dismutase - metabolism; 块茎; 慈姑; 抑制生长; 粗提取物; 脂质过氧化损伤; 诱导; 超氧化物歧化酶; 铜绿微囊藻
• ISSN: 1001-0742; 1878-7320
• DOI: 10.1016/j.jes.2015.08.020

Aquatic macrophytes are considered to be promising in controlling harmful cyanobacterial blooms. In this research, an aqueous extract of Sagittaria trifolia tubers was prepared to study its inhibitory effect on Microcystis aeruginosa in the laboratory. Several physiological indices of M. aeruginosa, in response to the environmental stress, were analyzed. Results showed that S. trifolia tuber aqueous extract significantly inhibited the growth of M. aeruginosa in a concentration-dependent way. The highest inhibition rate reached 90% after 6 day treatment. The Chlorophyll-a concentration of M. aeruginosa cells decreased from 343.1 to314.2 μg/L in the treatment group. The activities of superoxide dismutase and peroxidase and the content of reduced glutathione in M. aeruginosa cells initially increased as a response to the oxidative stress posed by S. trifolia tuber aqueous extract, but then decreased as time prolonged. The lipid peroxidation damage of the cyanobacterial cell membranes was reflected by the malondialdehyde level, which was notably higher in the treatment group compared with the controls. It was concluded that the oxidative damage of M. aeruginosa induced by S.trifolia tuber aqueous extract might be one of the mechanisms for the inhibitory effects.