Arctigenin reduces neuronal responses in the somatosensory cortex via the inhibition of non-NMDA glutamate receptors

• Published in: Neurochemistry international 2016-07, Vol.97, p.83-90
• Main Authors: Borbély, Sándor, Jócsák, Gergely, Moldován, Kinga, Sedlák, Éva, Preininger, Éva, Boldizsár, Imre, Tóth, Attila, Atlason, Palmi T., Molnár, Elek, Világi, Ildikó
• Format: Article
• Language: English
• Subjects: Animals; Arctigenin; Brain slices; Dose-Response Relationship, Drug; Evoked field potentials; Excitatory Amino Acid Antagonists - pharmacology; Furans - pharmacology; HEK293 Cells; Humans; In vivo experiments; Ionotropic glutamate receptors; Lignans - pharmacology; Male; Neurons - drug effects; Neurons - physiology; Organ Culture Techniques; Rat; Rats; Rats, Wistar; Receptors, Glutamate - physiology; Somatosensory Cortex - drug effects; Somatosensory Cortex - physiology
• ISSN: 0197-0186; 1872-9754
• DOI: 10.1016/j.neuint.2016.03.006

Lignans are biologically active phenolic compounds related to lignin, produced in different plants. Arctigenin, a dibenzylbutyrolactone-type lignan, has been used as a neuroprotective agent for the treatment of encephalitis. Previous studies of cultured rat cerebral cortical neurones raised the possibility that arctigenin inhibits kainate-induced excitotoxicity. The aims of the present study were: 1) to analyse the effect of arctigenin on normal synaptic activity in ex vivo brain slices, 2) to determine its receptor binding properties and test the effect of arctigenin on AMPA/kainate receptor activation and 3) to establish its effects on neuronal activity in vivo. Arctigenin inhibited glutamatergic transmission and reduced the evoked field responses. The inhibitory effect of arctigenin on the evoked field responses proved to be substantially dose dependent. Our results indicate that arctigenin exerts its effects under physiological conditions and not only on hyper-excited neurons. Furthermore, arctigenin can cross the blood–brain barrier and in the brain it interacts with kainate sensitive ionotropic glutamate receptors. These results indicate that arctigenin is a potentially useful new pharmacological tool for the inhibition of glutamate-evoked responses in the central nervous system in vivo. •Biologically active compounds, like arctigenin, a lignan produced by different plants may have neuroprotective effects.•Arctigenin effectively reduces normal, physiological electrical responses both in brain slices and in whole brain.•Arctigenin can cross the blood-brain barrier and it may exerts effect on nervous system of living animals.•Arctigenin effectively binds to GluK1 subunit of kainate receptors.•Different AMPA/KAIN types of ionotropic glutamate receptors may possess arctigenin binding properties.