Antioxidant and anti-aging activities of polysaccharides from Calocybe indica var. APK2

The crude polysaccharides were extracted from the fruiting bodies of Calocybe indica (CIP). The antioxidant activities of CIP were evaluated both in vitro and in vivo. Chemical characteristics of the polysaccharides were investigated. In in vitro antioxidant assay, CIP showed noticeable 2,2-diphenyl...

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Published in:Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2016-06, Vol.68 (6), p.329-334
Main Authors: Govindan, Sudha, Johnson, Elizabeth Elcy Rani, Christopher, Jabapramila, Shanmugam, Jayasakthi, Thirumalairaj, Vinothkumar, Gopalan, Jayanthi
Format: Article
Language:English
Subjects:
Agaricales - chemistry
Aging - drug effects
Animals
Anioxidants
Anti-aging
Antioxidants - chemistry
Antioxidants - pharmacology
Calocybe indica
Female
Fruiting Bodies, Fungal - chemistry
Fungal Polysaccharides - chemistry
Fungal Polysaccharides - pharmacology
Lipid peroxidation
Mice
Mice, Inbred BALB C
Polysaccharides
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Summary:The crude polysaccharides were extracted from the fruiting bodies of Calocybe indica (CIP). The antioxidant activities of CIP were evaluated both in vitro and in vivo. Chemical characteristics of the polysaccharides were investigated. In in vitro antioxidant assay, CIP showed noticeable 2,2-diphenyl-1-picryl-hydrazyl (DPPH), hydroxyl radical scavenging activities, reducing power and lipid peroxidation inhibition. Chemical analysis showed the presence of carbohydrate, protein and the FTIR spectra revealed the presence of general characteristic absorption peak of the polysaccharides. For in vivo antioxidant activity, two different doses of CIP were orally administrated over a period of 6 weeks in a d-galactose (d-gal) induced aged mice model. Significantly lowered activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), levels of glutathione (GSH) and elevated malondialdehyde (MDA) levels were observed in brain and serum of d-galactose induced rats, when compared to control rats. Administration of CIP significantly raised the activities of SOD, CAT, GPx, levels of GSH and lowered the levels of MDA in mice brain and serum in a dose-dependent manner. The results suggested that CIP had potent antioxidant activity and could minimize the occurrence of age-associated disorders associated with involvement of free radicals.
ISSN:0940-2993
1618-1433
DOI:10.1016/j.etp.2016.04.001