Thalassospira australica sp. nov. isolated from sea water

• Published in: Antonie van Leeuwenhoek 2016-08, Vol.109 (8), p.1091-1100
• Main Authors: Ivanova, Elena P., López-Pérez, Mario, Webb, Hayden K., Ng, Hooi Jun, Dang, Thi Hoang Yen, Zhukova, Natalia V., Mikhailov, Valery V., Crawford, Russell J., Rodriguez-Valera, Francisco
• Format: Article
• Language: English
• Subjects: Australia; Bacteria; Base Composition; Beaches; Biomedical and Life Sciences; Chemical analysis; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Fatty Acids - metabolism; Life Sciences; Medical Microbiology; Microbiology; Nucleic Acid Hybridization; Original Paper; Phylogeny; Plant Sciences; Rhodospirillaceae - classification; Rhodospirillaceae - genetics; Rhodospirillaceae - isolation & purification; Rhodospirillaceae - metabolism; RNA, Ribosomal, 16S - genetics; Seawater; Seawater - microbiology; Sequence Analysis, DNA; Sodium chloride; Soil Science & Conservation; Species Specificity; Water; Water analysis; Water sampling; Xiamenensis
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-016-0710-9

Two Gram-negative, non-pigmented, motile bacteria were isolated from a sea water sample collected at St. Kilda Beach, Port Philip Bay, Victoria, Australia. The two strains were found to grow between 4 and 40 °C, pH 5–10 and tolerate up to 10 % NaCl. A phylogenetic study, based on a 16S rRNA gene sequence analysis indicated that strains NP 3b2 T and H 94 belong to the genus Thalassospira . The sequence similarity of the 16S rRNA gene between the two new isolates is 99.8 % and between these strains and all validly named Thalassospira species was found to be in the range of 95–99.4 %. The DNA–DNA relatedness between the two strains was found to be 80.2 %, while relatedness with other validly named species of the genus Thalassospira was between 53 and 65 %. The average nucleotide identity (ANI) and the in silico genome-to-genome distance (GGD) between the two bacteria and T. profundimaris WP0211 T , T. xiamenensis M-5 T , ‘ T. permensis ’ NBRC 106175 T and T. lucentensis QMT2 T was 76–82 % and 21–25 %, respectively. The results of phylogenetic and genomic analysis, together with physiological and biochemical properties, indicated that the two strains represent a new species of the genus Thalassospira . Based on these data, a new species, Thalassospira australica , is proposed with strain NP 3b2 T (=KMM 6365 T  = JCM 31222 T ) as the type strain.