Enterococci isolated from farm ostriches and their relation to enterocins

• Published in: Folia microbiologica 2016-07, Vol.61 (4), p.275-281
• Main Authors: Laukova, Andrea, KandriAeakova, Anna, SAeerbova, Jana, Strompfova, Viola
• Format: Article
• Language: English
• Subjects: Animals; Animals, Domestic; Anti-Bacterial Agents - pharmacology; Antibiotics; Applied Microbiology; Bacteria; Bacterial Typing Techniques; Biomedical and Life Sciences; Birds; Bridged-Ring Compounds - metabolism; Enterococcus - classification; Enterococcus - drug effects; Enterococcus - genetics; Enterococcus - isolation & purification; Enterococcus faecium; Enterococcus hirae; Enterococcus mundtii; Environmental Engineering/Biotechnology; Feces; Feces - microbiology; Immunology; Life Sciences; Microbial Sensitivity Tests; Microbiology; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Struthioniformes - microbiology; Studies; Virulence Factors - analysis; Virulence Factors - genetics
• ISSN: 0015-5632; 1874-9356
• DOI: 10.1007/s12223-015-0435-6

The present study focuses on the detection of enterococci in ostrich faeces. Forty-six bacterial colonies from 140 ostriches were identified at the species level using the MALDI-TOF MS identification system. According to the score value evaluation, they were allotted to the species Enterococcus hirae , Enterococcus faecium and Enterococcus mundtii confirmed also by phenotypic testing. Dominated species E. hirae (34 strains) were submitted to more detailed testing. Those strains E. hirae produced either no or only slight amount of the enzymes related to disorders (N-acetyl-β-glucosaminidase, β-glucuronidase, α-chymotrypsin, trypsin). Most of the strains were not hemolytic. They did not harbour the hiracin-producing gene. Five E. hirae strains harboured virulence factor gene g elE ; however, they were phenotypically gelatinase negative. They also harboured other virulence factor genes such as esp , efaAfm and ccf. E. hirae strains were mostly sensitive to antibiotics and those resistant at least to one antibiotic were sensitive to enterocins (200–25,600 AU/mL). This study represents original and novel results concerning the enterococcal microflora in ostriches; enterococci in ostriches have not been described in detail up to now; sensitivity to enterocins of E. hirae strains harbouring virulence factor genes to enterocins is also new.