Dendritic cell-based vaccination with lentiviral vectors encoding ubiquitinated hepatitis B core antigen enhances hepatitis B virus-specific immune responses in vivo

The activity of hepatitis B virus （HBV）-specific cytotoxic T lymphocytes （CTLs） plays a predominant role in the clearance of HBV. Dendritic cells （DCs） are key antigen-presenting cells and play an import- ant role in the initiation of immune responses. We previously verified that lentiviral vector e...

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Bibliographic Details
Published in:Acta biochimica et biophysica Sinica 2015-11, Vol.47 (11), p.870-879
Main Authors: Dai, Shenglan, Zhuo, Meng, Song, Linlin, Chen, Xiaohua, Yu, Yongsheng, Tang, Zhenghao, Zang, Guoqing
Format: Article
Language:English
Subjects:
Animals
Dendritic Cells - immunology
Genetic Vectors - administration & dosage
Hepatitis B Core Antigens - chemistry
Hepatitis B Core Antigens - genetics
Hepatitis B Core Antigens - immunology
Hepatitis B Vaccines - genetics
Hepatitis B Vaccines - immunology
Hepatitis B virus
Lentivirus - genetics
Mice
Mice, Inbred BALB C
T-Lymphocytes, Cytotoxic - immunology
乙型肝炎病毒
免疫反应
异性
慢病毒载体
树突状细胞
核心抗原
泛素化
细胞疫苗
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Summary:The activity of hepatitis B virus （HBV）-specific cytotoxic T lymphocytes （CTLs） plays a predominant role in the clearance of HBV. Dendritic cells （DCs） are key antigen-presenting cells and play an import- ant role in the initiation of immune responses. We previously verified that lentiviral vector encoding ubiquitinated hepatitis B core antigen （LV-Ub-HBcAg） effectively transduced DCs to induce matur- ation, and the mature DCs efficiently induced T cell polarization to Thl and generated HBcAg-specific CTLs ex vivo. In this study, HBV-specific immune responses of LV-Ub-HBcAg in BALB/c mice （H-2Kd） were evaluated. It was shown that direct injection of LV-Ub-HBcAg increased the production of cyto- kines IL-2 and IFN-y, elicited strong antibody responses, and remarkably generated a high percent- age of IFN-y＋CD8＋ T cells with HBV-specific CTL responses in BALB/c mice. In addition, direct injection of LV-Ub-HBcAg induced potent anti-HBV immune responses, similar to those elicited by in vitro-transduced DCs. In conclusion, the DC-based therapeutic vaccine LV-Ub-HBcAg elicited spe- cific antibody immune responses and induced robust specific CTL activity in vivo.
ISSN:1672-9145
1745-7270
DOI:10.1093/abbs/gmv093