Synthesis of silver nanoparticles on the basis of low and high molar mass exopolysaccharides of Bradyrhizobium japonicum 36 and its antimicrobial activity against some pathogens

• Published in: Folia microbiologica 2016-07, Vol.61 (4), p.283-293
• Main Authors: Rasulov, Bakhtiyor, Rustamova, Nigora, Yili, Abulimiti, Zhao, Hai-Qing, Aisa, Haji A.
• Format: Article
• Language: English
• Subjects: Anti-Infective Agents - metabolism; Anti-Infective Agents - pharmacology; Antimicrobial agents; Applied Microbiology; Biomedical and Life Sciences; Bradyrhizobium - metabolism; Bradyrhizobium japonicum; Candida albicans; Candida albicans - drug effects; E coli; Environmental Engineering/Biotechnology; Escherichia coli; Escherichia coli - drug effects; Ethanol; Fourier transforms; Immunology; Infrared spectroscopy; Life Sciences; Microbial Sensitivity Tests; Microbiology; Microscopy, Electron, Transmission; Nanoparticles; Nanoparticles - chemistry; Nanoparticles - metabolism; Nanoparticles - ultrastructure; Oxidation-Reduction; Pathogens; Polysaccharides, Bacterial - metabolism; Silver; Silver - metabolism; Silver - pharmacology; Spectrophotometry; Spectroscopy, Fourier Transform Infrared; Staphylococcus aureus; Transmission electron microscopy; Ultraviolet spectroscopy; X-Ray Diffraction
• ISSN: 0015-5632; 1874-9356
• DOI: 10.1007/s12223-015-0436-5

Silver nanoparticles (SNPs) were synthesized on the basis of exopolysaccharides (low and high molar mass) of diazotrophic Bradyrhizobium japonicum 36 strain. The synthesis of SNPs was carried out by direct reduction of silver nitrate with ethanol-insoluble (high molar mass, HMW) and ethanol-soluble (low molar mass, LMW) fractions of exopolysaccharides (EPS), produced by diazotrophic strain B. japonicum 36. SNPs were characterized using UV-vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). SNPs synthesized on the basis of LMW EPS absorbed radiation in the visible regions of 420 nm, whereas SNPs based on the HMW EPS have a wavelength maximum at 450 nm because of the strong SPR transition. Moreover, the antibacterial and antifungal activities of the SNPs were examined in vitro against Escherichia coli , Staphylococcus aureus , and Candida albicans . SNPs synthesized on the basis of LMW EPS were active than those synthesized on the basis of HMW EPS. Besides, UV-visible spectroscopic evaluation confirmed that SNPs synthesized on the basis of LMW EPS were far more stable than those obtained on the basis of HMW EPS.