Lentiviral Transduction of Neurons in Adult Brain: Evaluation of Inflammatory Response and Cognitive Effects in Mice

We evaluated the effect of hippocampal injection of lentiviral particles p156-CMV-EGFP on behavior, learning, and microglial Iba1 + cells activation in mice. Testing in the open field and elevated plus-maze revealed higher anxiety levels in lentiviral-injected mice in comparison with animals injecte...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2016-06, Vol.161 (2), p.316-319
Main Authors: Kunitsyna, T. A., Ivashkina, O. I., Roshchina, M. A., Toropova, K. A., Anokhin, K. V.
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
CA1 Region, Hippocampal - immunology
CA1 Region, Hippocampal - virology
Cell Biology
Cognition
Dentate Gyrus - immunology
Dentate Gyrus - virology
Inflammation
Internal Medicine
Laboratory Medicine
Lentivirus
Lentivirus - genetics
Lentivirus - immunology
Male
Maze Learning
Mice, Inbred C57BL
Neurons
Neurons - immunology
Neurons - virology
Neurophysiology
Pathology
Transduction, Genetic
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Summary:We evaluated the effect of hippocampal injection of lentiviral particles p156-CMV-EGFP on behavior, learning, and microglial Iba1 + cells activation in mice. Testing in the open field and elevated plus-maze revealed higher anxiety levels in lentiviral-injected mice in comparison with animals injected with vehicle. At the same time, lentivirus injection did not change learning and memory of mice in the hippocampal-dependent fear conditioning task. Microglia density in lentivirus-injected mice was significantly higher than in vehicle-injected mice. Thus, hippocampal injection of lentiviral particles with minimum content of transgenes produced evident inflammation process, changed anxiety level of experimental animals, but had no effect on hippocampal-dependent learning and memory.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-016-3404-4