An effective, rapid and simple method for total RNA extraction from bacteria and yeast

In this work, we describe a rapid and simple method for total RNA extraction from bacteria and yeast. The method allows for the acquirement of high RNA yields while avoiding the use of phenol or other toxic reagents and is less expensive than other methods previously described. The extracted RNA is...

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Bibliographic Details
Published in:Journal of microbiological methods 2001-10, Vol.47 (1), p.59-63
Main Authors: Rivas, Raúl, Vizcaı́no, Nieves, Buey, Rubén M, Mateos, Pedro F, Martı́nez-Molina, Eustoquio, Velázquez, Encarna
Format: Article
Language:English
Subjects:
Bacteria
Bacteria - genetics
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Blotting, Northern
Electrophoresis
Electrophoresis - methods
Extraction
Fundamental and applied biological sciences. Psychology
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Microbiology
Mycology
Reverse Transcriptase Polymerase Chain Reaction
RNA
RNA, Bacterial - analysis
RNA, Bacterial - isolation & purification
RNA, Fungal - analysis
RNA, Fungal - isolation & purification
Time Factors
Yeast
Yeasts - genetics
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Summary:In this work, we describe a rapid and simple method for total RNA extraction from bacteria and yeast. The method allows for the acquirement of high RNA yields while avoiding the use of phenol or other toxic reagents and is less expensive than other methods previously described. The extracted RNA is suitable for applications such as RT-PCR, Northern blot hybridization and low molecular weight RNA (LMW RNA) electrophoresis.
ISSN:0167-7012
1872-8359
DOI:10.1016/S0167-7012(01)00292-5