Analysis of Kynurenine/Tryptophan ratio and expression of IDO1 and 2 mRNA in tumour tissue of cervical cancer patients

Indoleamine 2,3-Dioxygenase (IDO) catalyses the degradation of the essential amino acid tryptophan leading to the production of immunosuppressive Kynurenine. In the present study, we developed a modified method for measurement of Kynurenine/tryptophan (K/T) ratio in the cervical tissue using HPLC an...

Full description

Saved in:
Bibliographic Details
Published in:Clinical biochemistry 2016-08, Vol.49 (12), p.919-924
Main Authors: Hascitha, Jayakumar, Priya, Ramanathan, Jayavelu, Subramani, Dhandapani, Hemavathi, Selvaluxmy, Ganeshrajah, Sunder Singh, Shirley, Rajkumar, Thangarajan
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - metabolism
Carcinoma, Squamous Cell - pathology
Case-Control Studies
Cervical cancer
Chromatography, High Pressure Liquid
Female
Follow-Up Studies
HPLC
Humans
IDO
Indoleamine-Pyrrole 2,3,-Dioxygenase - genetics
Kynurenine
Kynurenine - metabolism
Middle Aged
Neoplasm Grading
Neoplasm Staging
Prognosis
Real-Time Polymerase Chain Reaction
RNA, Messenger - genetics
Survival Rate
Tryptophan
Tryptophan - metabolism
Uterine Cervical Neoplasms - genetics
Uterine Cervical Neoplasms - metabolism
Uterine Cervical Neoplasms - pathology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Indoleamine 2,3-Dioxygenase (IDO) catalyses the degradation of the essential amino acid tryptophan leading to the production of immunosuppressive Kynurenine. In the present study, we developed a modified method for measurement of Kynurenine/tryptophan (K/T) ratio in the cervical tissue using HPLC and investigated its relationship with the expression of IDO1 and 2 genes in the cervical tumour milieu. Cervical cancer punch biopsy samples of 27 women who presented at the Cancer Institute (WIA) were used for detection of K/T ratio by HPLC as well as expression of IDO1 and 2 at the mRNA level by Realtime PCR after obtaining Institutional ethical committee approval. The K/T ratio was elevated significantly in cancer cervix samples compared to normal cervix (p
ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2016.04.008