Comparison of single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods for detection of Myxobolus cerebralis

Single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods were compared for the detection of Myxobolus cerebralis. Parallel tests were conducted on a total of 1743 free-ranging and 400 hatchery-reared salmonids. Concurrent results were found in 84.6% of the free-ranging fi...

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Bibliographic Details
Published in:Diseases of aquatic organisms 2000-06, Vol.45 (2), p.109-114
Main Authors: Schisler, G J, Bergersen, E P, Walker, P G, Wood, J, Epp, J K
Format: Article
Language:English
Subjects:
Brackish
Freshwater
Marine
Myxobolus cerebralis
Salmonidae
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Summary:Single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods were compared for the detection of Myxobolus cerebralis. Parallel tests were conducted on a total of 1743 free-ranging and 400 hatchery-reared salmonids. Concurrent results were found in 84.6% of the free-ranging fish samples, and 83.5% of the hatchery samples. PCR identified M. cerebralis more frequently than did PTD, and did so in many geographic locations previously considered free of the parasite. Average myxospore count by PTD among both free-ranging and hatchery fish increased significantly (p < 0.001) with a subjective evaluation of amplicon staining intensity.
ISSN:0177-5103
1616-1580