Comparison of single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods for detection of Myxobolus cerebralis

Single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods were compared for the detection of Myxobolus cerebralis. Parallel tests were conducted on a total of 1743 free-ranging and 400 hatchery-reared salmonids. Concurrent results were found in 84.6% of the free-ranging fi...

Full description

Saved in:
Bibliographic Details
Published in:Diseases of aquatic organisms 2000-06, Vol.45 (2), p.109-114
Main Authors: Schisler, G J, Bergersen, E P, Walker, P G, Wood, J, Epp, J K
Format: Article
Language:English
Subjects:
Brackish
Freshwater
Marine
Myxobolus cerebralis
Salmonidae
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 114
container_issue 2
container_start_page 109
container_title Diseases of aquatic organisms
container_volume 45
creator Schisler, G J
Bergersen, E P
Walker, P G
Wood, J
Epp, J K
description Single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods were compared for the detection of Myxobolus cerebralis. Parallel tests were conducted on a total of 1743 free-ranging and 400 hatchery-reared salmonids. Concurrent results were found in 84.6% of the free-ranging fish samples, and 83.5% of the hatchery samples. PCR identified M. cerebralis more frequently than did PTD, and did so in many geographic locations previously considered free of the parasite. Average myxospore count by PTD among both free-ranging and hatchery fish increased significantly (p < 0.001) with a subjective evaluation of amplicon staining intensity.
format article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_18095117</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>18095117</sourcerecordid><originalsourceid>FETCH-proquest_miscellaneous_180951173</originalsourceid><addsrcrecordid>eNqNj8FOwzAQRC0EEqHwD3tC9GDJ2ypNew4gLkio6r1yk01r5HhTryORP-CzcQUfwGkO82ZGc6UKXOFKY7k216owWFW6RLO8VXcin8bgYlNiob5r7gcbnXAA7kBcOHrSkcfQwsB-6ilaIWhO1gWIZJvkMvn0UW_nYC8MDTmjU5wuCq07kqTs757n0FM6cSvQcYSWEv1m88r79MUH9qNAQ5EO0Xon9-qms17o4U9n6vH1ZVe_6SHyecyl-95JQ97bQDzKHtcmH8Bq-W_wBzpNV-k</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18095117</pqid></control><display><type>article</type><title>Comparison of single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods for detection of Myxobolus cerebralis</title><source>Freely Accessible Journals</source><creator>Schisler, G J ; Bergersen, E P ; Walker, P G ; Wood, J ; Epp, J K</creator><creatorcontrib>Schisler, G J ; Bergersen, E P ; Walker, P G ; Wood, J ; Epp, J K</creatorcontrib><description>Single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods were compared for the detection of Myxobolus cerebralis. Parallel tests were conducted on a total of 1743 free-ranging and 400 hatchery-reared salmonids. Concurrent results were found in 84.6% of the free-ranging fish samples, and 83.5% of the hatchery samples. PCR identified M. cerebralis more frequently than did PTD, and did so in many geographic locations previously considered free of the parasite. Average myxospore count by PTD among both free-ranging and hatchery fish increased significantly (p &lt; 0.001) with a subjective evaluation of amplicon staining intensity.</description><identifier>ISSN: 0177-5103</identifier><identifier>EISSN: 1616-1580</identifier><language>eng</language><subject>Brackish ; Freshwater ; Marine ; Myxobolus cerebralis ; Salmonidae</subject><ispartof>Diseases of aquatic organisms, 2000-06, Vol.45 (2), p.109-114</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Schisler, G J</creatorcontrib><creatorcontrib>Bergersen, E P</creatorcontrib><creatorcontrib>Walker, P G</creatorcontrib><creatorcontrib>Wood, J</creatorcontrib><creatorcontrib>Epp, J K</creatorcontrib><title>Comparison of single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods for detection of Myxobolus cerebralis</title><title>Diseases of aquatic organisms</title><description>Single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods were compared for the detection of Myxobolus cerebralis. Parallel tests were conducted on a total of 1743 free-ranging and 400 hatchery-reared salmonids. Concurrent results were found in 84.6% of the free-ranging fish samples, and 83.5% of the hatchery samples. PCR identified M. cerebralis more frequently than did PTD, and did so in many geographic locations previously considered free of the parasite. Average myxospore count by PTD among both free-ranging and hatchery fish increased significantly (p &lt; 0.001) with a subjective evaluation of amplicon staining intensity.</description><subject>Brackish</subject><subject>Freshwater</subject><subject>Marine</subject><subject>Myxobolus cerebralis</subject><subject>Salmonidae</subject><issn>0177-5103</issn><issn>1616-1580</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqNj8FOwzAQRC0EEqHwD3tC9GDJ2ypNew4gLkio6r1yk01r5HhTryORP-CzcQUfwGkO82ZGc6UKXOFKY7k216owWFW6RLO8VXcin8bgYlNiob5r7gcbnXAA7kBcOHrSkcfQwsB-6ilaIWhO1gWIZJvkMvn0UW_nYC8MDTmjU5wuCq07kqTs757n0FM6cSvQcYSWEv1m88r79MUH9qNAQ5EO0Xon9-qms17o4U9n6vH1ZVe_6SHyecyl-95JQ97bQDzKHtcmH8Bq-W_wBzpNV-k</recordid><startdate>20000601</startdate><enddate>20000601</enddate><creator>Schisler, G J</creator><creator>Bergersen, E P</creator><creator>Walker, P G</creator><creator>Wood, J</creator><creator>Epp, J K</creator><scope>7TN</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20000601</creationdate><title>Comparison of single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods for detection of Myxobolus cerebralis</title><author>Schisler, G J ; Bergersen, E P ; Walker, P G ; Wood, J ; Epp, J K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_180951173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Brackish</topic><topic>Freshwater</topic><topic>Marine</topic><topic>Myxobolus cerebralis</topic><topic>Salmonidae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schisler, G J</creatorcontrib><creatorcontrib>Bergersen, E P</creatorcontrib><creatorcontrib>Walker, P G</creatorcontrib><creatorcontrib>Wood, J</creatorcontrib><creatorcontrib>Epp, J K</creatorcontrib><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Diseases of aquatic organisms</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schisler, G J</au><au>Bergersen, E P</au><au>Walker, P G</au><au>Wood, J</au><au>Epp, J K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods for detection of Myxobolus cerebralis</atitle><jtitle>Diseases of aquatic organisms</jtitle><date>2000-06-01</date><risdate>2000</risdate><volume>45</volume><issue>2</issue><spage>109</spage><epage>114</epage><pages>109-114</pages><issn>0177-5103</issn><eissn>1616-1580</eissn><abstract>Single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods were compared for the detection of Myxobolus cerebralis. Parallel tests were conducted on a total of 1743 free-ranging and 400 hatchery-reared salmonids. Concurrent results were found in 84.6% of the free-ranging fish samples, and 83.5% of the hatchery samples. PCR identified M. cerebralis more frequently than did PTD, and did so in many geographic locations previously considered free of the parasite. Average myxospore count by PTD among both free-ranging and hatchery fish increased significantly (p &lt; 0.001) with a subjective evaluation of amplicon staining intensity.</abstract></addata></record>
fulltext fulltext
identifier ISSN: 0177-5103
ispartof Diseases of aquatic organisms, 2000-06, Vol.45 (2), p.109-114
issn 0177-5103
1616-1580
language eng
recordid cdi_proquest_miscellaneous_18095117
source Freely Accessible Journals
subjects Brackish
Freshwater
Marine
Myxobolus cerebralis
Salmonidae
title Comparison of single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods for detection of Myxobolus cerebralis
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T21%3A19%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20single-round%20polymerase%20chain%20reaction%20(PCR)%20and%20pepsin-trypsin%20digest%20(PTD)%20methods%20for%20detection%20of%20Myxobolus%20cerebralis&rft.jtitle=Diseases%20of%20aquatic%20organisms&rft.au=Schisler,%20G%20J&rft.date=2000-06-01&rft.volume=45&rft.issue=2&rft.spage=109&rft.epage=114&rft.pages=109-114&rft.issn=0177-5103&rft.eissn=1616-1580&rft_id=info:doi/&rft_dat=%3Cproquest%3E18095117%3C/proquest%3E%3Cgrp_id%3Ecdi_FETCH-proquest_miscellaneous_180951173%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=18095117&rft_id=info:pmid/&rfr_iscdi=true