Multi-layered, membrane-integrated microfluidics based on replica molding of a thiol-ene epoxy thermoset for organ-on-a-chip applications

In this study we have investigated a photosensitive thermoset (OSTEMER 322-40) as a complementary material to readily fabricate complex multi-layered microdevices for applications in life science. Simple, versatile and robust fabrication of multifunctional microfluidics is becoming increasingly impo...

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Bibliographic Details
Published in:Lab on a chip 2015-01, Vol.15 (24), p.4542-4554
Main Authors: Sticker, Drago, Rothbauer, Mario, Lechner, Sarah, Hehenberger, Marie-Therese, Ertl, Peter
Format: Article
Language:English
Subjects:
Animals
Barriers
Biocompatible Materials - chemistry
Biological
Cell Line
Cell Survival
Dimethylpolysiloxanes - chemistry
Epithelial Cells - cytology
Epoxy Compounds - chemistry
Equipment Design
Fibroblasts - cytology
Fluorescence
Fluoropolymers
Humans
Lab-On-A-Chip Devices
Membranes
Mesenchymal Stromal Cells - cytology
Mice
Microfluidic Analytical Techniques - instrumentation
Microfluidics
Micropumps
NIH 3T3 Cells
Organ Culture Techniques - instrumentation
Sulfhydryl Compounds - chemistry
Thermosetting resins
Three dimensional
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Summary:In this study we have investigated a photosensitive thermoset (OSTEMER 322-40) as a complementary material to readily fabricate complex multi-layered microdevices for applications in life science. Simple, versatile and robust fabrication of multifunctional microfluidics is becoming increasingly important for the development of customized tissue-, organ- and body-on-a-chip systems capable of mimicking tissue interfaces and biological barriers. In the present work key material properties including optical properties, vapor permeability, hydrophilicity and biocompatibility are evaluated for cell-based assays using fibroblasts, endothelial cells and mesenchymal stem cells. The excellent bonding strength of the OSTEMER thermoset to flexible fluoropolymer (FEP) sheets and poly(dimethylsiloxane) (PDMS) membranes further allows for the fabrication of integrated microfluidic components such as membrane-based microdegassers, microvalves and micropumps. We demonstrate the application of multi-layered, membrane-integrated microdevices that consist of up to seven layers and three membranes that specially confine and separate vascular cells from the epithelial barrier and 3D tissue structures.
ISSN:1473-0197
1473-0189
DOI:10.1039/c5lc01028d