Age-Related Changes in the Function and Structure of the Peripheral Sensory Pathway in Mice

Abstract This study is aimed at describing the changes occurring in the entire peripheral nervous system (PNS) sensory pathway along a 2-year observation period in a cohort of C57BL/6 mice. The neurophysiological studies evidenced significant differences in the selected time points corresponding to...

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Bibliographic Details
Published in:Neurobiology of aging 2016-09, Vol.45, p.136-148
Main Authors: Canta, A, Chiorazzi, A, Carozzi, V, Meregalli, C, Oggioni, N, Bossi, M, Rodriguez-Menendez, V, Avezza, F, Crippa, L, Lombardi, R, de Vito, G, Piazza, V, Cavaletti, G, Marmiroli, P
Format: Article
Language:English
Subjects:
Aging
Aging - pathology
Aging - physiology
Animals
Female
Ganglia, Spinal - pathology
Ganglia, Spinal - physiology
Ganglia, Spinal - physiopathology
Internal Medicine
Mice
Mice, Inbred C57BL
Morphometric analysis
Nerve conduction velocity
Neural Conduction - physiology
Neural Pathways - pathology
Neural Pathways - physiology
Neural Pathways - physiopathology
Neurology
Peripheral nervous system
Peripheral Nervous System - pathology
Peripheral Nervous System - physiology
Peripheral Nervous System - physiopathology
RP-CARS
Skin - innervation
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Summary:Abstract This study is aimed at describing the changes occurring in the entire peripheral nervous system (PNS) sensory pathway along a 2-year observation period in a cohort of C57BL/6 mice. The neurophysiological studies evidenced significant differences in the selected time points corresponding to childhood, young adulthood, adulthood and aging (i.e. 1, 7, 15 and 25 month of age), with a parabolic course as function of time. The pathological assessment allowed to demonstrate signs of age-related changes since the age of 7 months, with a remarkable increase in both peripheral nerves and dorsal root ganglia (DRG) at the subsequent time points. These changes were mainly in the myelin sheaths, as also confirmed by the Rotating-Polarization Coherent-Anti-stokes-Raman-scattering (RP-CARS) microscopy analysis. Evident changes were also present at the morphometric analysis performed on the peripheral nerves, DRG neurons and skin biopsies. This extensive, multimodal characterization of the PNS changes in aging provides the background for future mechanistic studies allowing the selection of the most appropriate time points and readouts according to the investigation aims.
ISSN:0197-4580
1558-1497
DOI:10.1016/j.neurobiolaging.2016.05.014