NF-κB Binds P-TEFb to Stimulate Transcriptional Elongation by RNA Polymerase II

To stimulate transcriptional elongation of HIV-1 genes, the transactivator Tat recruits the positive transcription elongation factor b (P-TEFb) to the initiating RNA polymerase II (RNAPII). We found that the activation of transcription by RelA also depends on P-TEFb. Similar to Tat, RelA activated t...

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Bibliographic Details
Published in:Molecular cell 2001-08, Vol.8 (2), p.327-337
Main Authors: Barboric, Matjaz, Nissen, Robert M., Kanazawa, Satoshi, Jabrane-Ferrat, Nabila, Peterlin, B.Matija
Format: Article
Language:English
Subjects:
Human immunodeficiency virus 1
Il-8 gene
NF-B protein
pol II protein
RelA protein
transcription elongation factor b
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Summary:To stimulate transcriptional elongation of HIV-1 genes, the transactivator Tat recruits the positive transcription elongation factor b (P-TEFb) to the initiating RNA polymerase II (RNAPII). We found that the activation of transcription by RelA also depends on P-TEFb. Similar to Tat, RelA activated transcription when tethered to RNA. Moreover, TNF-α triggered the recruitment of P-TEFb to the NF-κB-regulated IL-8 gene. While the formation of the transcription preinitiation complex (PIC) remained unaffected, DRB, an inhibitor of P-TEFb, prevented RNAPII from elongating on the IL-8 gene. Remarkably, DRB inhibition sensitized cells to TNF-α-induced apoptosis. Thus, NF-κB requires P-TEFb to stimulate the elongation of transcription and P-TEFb plays an unexpected role in regulating apoptosis.
ISSN:1097-2765
1097-4164
DOI:10.1016/S1097-2765(01)00314-8