Inflammatory Profile in Response to Uncontrolled Hemorrhage in a Non-Human Primate (Rhesus Macaque) Model

BACKGROUND:Uncontrolled hemorrhage (UH), the leading cause of potentially survivable combat-related death, elicits a deleterious inflammatory response. Our group previously reported an increased secretion of pro-inflammatory cytokines in a novel non-human primate model of UH; however, to better unde...

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Bibliographic Details
Published in:Shock (Augusta, Ga.) Ga.), 2016-09, Vol.46 (3S Suppl 1), p.115-122
Main Authors: Burdette, Alexander J, Paredes, Ruth Madelaine, Crossland, Randy F, Macko, Antoni R, Aden, James, Sheppard, Forest R
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Chemokines - genetics
Chemokines - metabolism
Cytokines - genetics
Cytokines - metabolism
Disease Models, Animal
Gene Expression - genetics
Hemorrhage - genetics
Hemorrhage - immunology
Hemorrhage - metabolism
Inflammation - genetics
Inflammation - metabolism
Macaca mulatta - genetics
Macaca mulatta - immunology
Macaca mulatta - metabolism
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Summary:BACKGROUND:Uncontrolled hemorrhage (UH), the leading cause of potentially survivable combat-related death, elicits a deleterious inflammatory response. Our group previously reported an increased secretion of pro-inflammatory cytokines in a novel non-human primate model of UH; however, to better understand the molecular profile of the inflammatory response to UH, we performed a comprehensive evaluation of inflammation at the proteomic and transcriptomic level. METHODS:Anesthetized rhesus macaques (n = 8) underwent UH by 60% left lobe hepatectomy T = 0 min. At T = 5 min, animals received 11 mL of 5% albumin followed by normal saline infusion to a total resuscitation volume of 20 mL/kg by T = 120 min. Blood (T = 0, 5, 20, 120, 480 min) was collected for qPCR and multiplex cytokine quantification. Results from each non-human primate (NHP) per time-point are shown. Statistical analysis by one-way ANOVA with repeated measures, P 
ISSN:1073-2322
1540-0514
DOI:10.1097/SHK.0000000000000638