Identification and Quantification of Loline-Type Alkaloids in Endophyte-Infected Grasses by LC-MS/MS

Lolines, fungal metabolites of the grass–endophyte association, were identified and quantified using newly developed LC-MS/MS methods in endophyte-infected grasses belonging to the Lolium and Festuca genera after extraction with three different solvents using two extraction methods. The shaking extr...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2016-08, Vol.64 (31), p.6212-6218
Main Authors: Adhikari, Khem B., Boelt, Birte, Fomsgaard, Inge S.
Format: Article
Language:English
Subjects:
Alkaloids - chemistry
Alkaloids - metabolism
Chromatography, Liquid - methods
Endophytes - metabolism
Festuca - chemistry
Festuca - microbiology
Fungi - metabolism
Lolium - chemistry
Lolium - microbiology
Poaceae - chemistry
Poaceae - microbiology
Tandem Mass Spectrometry - methods
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Summary:Lolines, fungal metabolites of the grass–endophyte association, were identified and quantified using newly developed LC-MS/MS methods in endophyte-infected grasses belonging to the Lolium and Festuca genera after extraction with three different solvents using two extraction methods. The shaking extraction method with isopropanol/water was superior to the other methods due to its high sensitivity, high accuracy (recovery within or close to the range of 80–120%), and high precision (coefficient of variation of
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.6b02616