Identification and Quantification of Loline-Type Alkaloids in Endophyte-Infected Grasses by LC-MS/MS

Lolines, fungal metabolites of the grass–endophyte association, were identified and quantified using newly developed LC-MS/MS methods in endophyte-infected grasses belonging to the Lolium and Festuca genera after extraction with three different solvents using two extraction methods. The shaking extr...

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Published in:Journal of agricultural and food chemistry 2016-08, Vol.64 (31), p.6212-6218
Main Authors: Adhikari, Khem B., Boelt, Birte, Fomsgaard, Inge S.
Format: Article
Language:English
Subjects:
Alkaloids - chemistry
Alkaloids - metabolism
Chromatography, Liquid - methods
Endophytes - metabolism
Festuca - chemistry
Festuca - microbiology
Fungi - metabolism
Lolium - chemistry
Lolium - microbiology
Poaceae - chemistry
Poaceae - microbiology
Tandem Mass Spectrometry - methods
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cited_by cdi_FETCH-LOGICAL-a336t-f1495c1a6e9d4a18b403ce60f10a5ac87931361dd3be315e428df7c71580f0a93
cites cdi_FETCH-LOGICAL-a336t-f1495c1a6e9d4a18b403ce60f10a5ac87931361dd3be315e428df7c71580f0a93
container_end_page 6218
container_issue 31
container_start_page 6212
container_title Journal of agricultural and food chemistry
container_volume 64
creator Adhikari, Khem B.
Boelt, Birte
Fomsgaard, Inge S.
description Lolines, fungal metabolites of the grass–endophyte association, were identified and quantified using newly developed LC-MS/MS methods in endophyte-infected grasses belonging to the Lolium and Festuca genera after extraction with three different solvents using two extraction methods. The shaking extraction method with isopropanol/water was superior to the other methods due to its high sensitivity, high accuracy (recovery within or close to the range of 80–120%), and high precision (coefficient of variation of
doi_str_mv 10.1021/acs.jafc.6b02616
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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Alkaloids - chemistry
Alkaloids - metabolism
Chromatography, Liquid - methods
Endophytes - metabolism
Festuca - chemistry
Festuca - microbiology
Fungi - metabolism
Lolium - chemistry
Lolium - microbiology
Poaceae - chemistry
Poaceae - microbiology
Tandem Mass Spectrometry - methods
title Identification and Quantification of Loline-Type Alkaloids in Endophyte-Infected Grasses by LC-MS/MS
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