Structure and antioxidative property of a polysaccharide from an ammonium oxalate extract of Phellinus linteus

• Published in: International journal of biological macromolecules 2016-10, Vol.91, p.92-99
• Main Authors: Yan, Jing-Kun, Wang, Yao-Yao, Wang, Zhen-Bin, Ma, Hai-Le, Pei, Juan-Juan, Wu, Jian-Yong
• Format: Article
• Language: English
• Subjects: Aging - metabolism; Animals; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Antioxidative activity; Female; Malondialdehyde - metabolism; Mice; Mice, Inbred ICR; Molecular Weight; Monosaccharides - analysis; Oxalic Acid - chemistry; Phellinus linteus polysaccharide; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Structural characterization; Structure-Activity Relationship; Viscera - drug effects; Viscera - metabolism
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2016.05.063

•A novel polysaccharide (PL-A11) purified from acidic extract of P. linteus mycelia.•PL-A11 was a neutral heteropolysaccharide with an Mw of 13.8kDa.•The structural feature of PL-A11 was elucidated by physical and chemical analyses.•PL-A11 exhibited potential antioxidant activities in vivo.•PL-A11 could be developed as a functional food or therapeutic agent. In this paper, the novel polysaccharide PL-A11 was purified from an ammonium oxalate extract of Phellinus linteus mycelia. Its physicochemical properties, structural characteristics, and antioxidant activities were investigated. Results showed that PL-A11 had a weight-average molecular weight (Mw) of 13.8kDa and was mainly composed of arabinose, xylose, mannose, and glucose in a molar ratio of 1.1:1.3:1.0:6.6. The backbone of PL-A11 was composed of (1→4)-α-d-glucopyranosyl, (1→2)-α-d-xylopyranosyl, and (1→3)-α-d-arabinofuranosyl residues, whereas the (1→6)-α-d-mannopyranosyl residues formed branches at the O-2 position with 1-linked-α-d-glucopyranosyl terminal residues. From the antioxidative activity tests in vivo, the administration of PL-A11 obviously enhanced the activity of antioxidant enzymes and significantly reduced the level of malondiadehyde (MDA) in the serum and liver of d-galactose-treated aging mice in a dose-dependent manner, as well as effectively stimulated the immune system of aging mice. These findings implied that PL-A11 could be developed as a potential antioxidant for applications in the functional food, pharmaceutical, cosmetic or nutraceutical industries.