Enzyme coated beta-cyclodextrin for effective adsorption and glucose-responsive closed-loop insulin delivery

[Display omitted] ⿢Synthesized a novel drug carrier for simultaneous glucose sensing and insulin delivery.⿢Prepared material facilitates efficient entrapment of insulin owing to the increased hydrophobicity of beta-cyclodextrin.⿢The biological effectiveness was assessed using flow cytometry analysis...

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Bibliographic Details
Published in:International journal of biological macromolecules 2016-10, Vol.91, p.818-827
Main Authors: Anirudhan, T.S., Nair, Anoop S., Nair, Syam S.
Format: Article
Language:English
Subjects:
beta-Cyclodextrins - chemistry
beta-Cyclodextrins - pharmacology
Closed loop-insulin delivery
Drug Delivery Systems
Glucose - chemistry
Glucose - pharmacology
Glucose Oxidase - chemistry
Glucose Oxidase - pharmacology
Glucose oxidase-catalase
HeLa Cells
Humans
Hydrophobic beta cyclodextrin
Insulin - chemistry
Insulin - pharmacokinetics
Insulin - pharmacology
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Summary:[Display omitted] ⿢Synthesized a novel drug carrier for simultaneous glucose sensing and insulin delivery.⿢Prepared material facilitates efficient entrapment of insulin owing to the increased hydrophobicity of beta-cyclodextrin.⿢The biological effectiveness was assessed using flow cytometry analysis.⿢Prepared material can act as a potential substitute for conventional forms of insulin administration. Inconsistent dosage of insulin (INS) for type 2 diabetes patients lead to severe adverse effects like limb amputation, blindness and fatal hypo or hyper glycaemia. Hence, a drug delivery system (DDS) capable of consistent INS release by sensing changes in blood glucose level is essential. Herein, we report a glucose responsive DDS comprised of oleic acid-grafted-aminated beta cyclodextrin (OA-g-ACD) copolymer, coated with a dispersion of glucose oxidase (GOx) and catalase (CAT). The prepared DDS was characterised using FTIR, Optical Microscopy, H1 NMR, DLS and SEM. Hydrophobicity and drug loading capacity was ascertained using contact angle measurements and confocal laser scanning microscopy (CLSM) respectively. Extent of swelling was observed to be a function of glucose concentration. INS release profile showed a cumulative release of 78.0 % after 240min. Flow cytometry studies revealed greater population of INS on HeLa cells indicating application of DDS as potential candidate for the intravenous administration of INS.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2016.06.028