Chemical composition and in vitro activity of Calotropis procera (Ait.) latex on Haemonchus contortus

•A new compound, 1-(2′,5′-dimethoxyphenyl)-glycerol, was isolated in ethyl acetate extract of Calotropis procera latex (EAECPL).•EAECPL efficiently inhibited egg hatching, larval development and motility of the adult worms of Haemonchus contortus.•Structural changes were not detected in the worm...

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Published in:Veterinary parasitology 2016-08, Vol.226, p.22-25
Main Authors: Cavalcante, Géssica S., de Morais, Selene M., Andre, Weibson P.P., Ribeiro, Wesley L.C., Rodrigues, Ana L.M., De Lira, Fábia C.M.L., Viana, Janaína M., Bevilaqua, Claudia M.L.
Format: Article
Language:English
Subjects:
Abomasum - parasitology
Acetates
Animals
Calotropis - chemistry
Calotropis procera
Feces - parasitology
Freeze Drying - veterinary
Gastrointestinal nematodes
Haemonchiasis - drug therapy
Haemonchiasis - parasitology
Haemonchiasis - veterinary
Haemonchus - drug effects
Haemonchus - growth & development
Haemonchus - physiology
Haemonchus - ultrastructure
Latex - chemistry
Latex - pharmacology
Magnetic Resonance Spectroscopy
Microscopy, Electron, Scanning - veterinary
Parasite Egg Count - veterinary
Phytotherapy
Plant Extracts - chemistry
Plant Extracts - pharmacology
Sheep
Sheep Diseases - drug therapy
Sheep Diseases - parasitology
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Summary:•A new compound, 1-(2′,5′-dimethoxyphenyl)-glycerol, was isolated in ethyl acetate extract of Calotropis procera latex (EAECPL).•EAECPL efficiently inhibited egg hatching, larval development and motility of the adult worms of Haemonchus contortus.•Structural changes were not detected in the worm's cuticle treated with EAECPL. Calotropis procera is among the species of medicinal plants that have traditionally been used for the treatment of parasites in small ruminants, stimulating the scientific validation of anthelmintic effects. This study aimed to investigate the chemical composition of ethyl acetate extract of Calotropis procera latex (EAECPL), assess the in vitro effect against Haemonchus contortus and the structural changes caused in the adult worm. The latex was collected, lyophilized and subjected to washing with the ethyl acetate solvent to obtain EAECPL. The constituents of the extract were isolated by column chromatography and identified by 13C and 1H nuclear magnetic resonance spectroscopy. The egg hatching test (EHT), larval development test (LDT) and adult worms motility test (WMT) were conducted to evaluate the effectiveness of EAECPL on eggs, larvae and adult of H. contortus, respectively. The worms obtained from the WMT, after 24h exposure to EAECPL or controls were observed on a scanning electron microscope (SEM). The results were analysed by variance analysis and compared with Tukey’s test (P
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2016.06.012