The role of glucose in the Kluyveromyces bulgaricus flocculation phenomenon: transduction by cAMP-dependent protein kinase pathway?

Yeast flocculation appears to be dependent on several culture conditions such as nitrogen or carbon sources. In 0.2% glucose medium Kluyveromyces bulgaricus flocculation intensity is weak (10% at maximum) by comparison with flocculation in 2% glucose medium (85% maximum). Addition of glucose to K. b...

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Bibliographic Details
Published in:FEMS microbiology letters 2001-09, Vol.203 (2), p.229-233
Main Authors: Géhin, Gérald, Bonaly, Roger, Coulon, Joël
Format: Article
Language:English
Subjects:
2,4-Dinitrophenol - pharmacology
Bacterial diseases
Bacteriology
Biological and medical sciences
Culture Media
Cyclic AMP - pharmacology
Cyclic AMP-Dependent Protein Kinases - antagonists & inhibitors
Cyclic AMP-Dependent Protein Kinases - metabolism
Cycloheximide - pharmacology
Experimental bacterial diseases and models
Flocculation
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Fungal
Glucose
Glucose - metabolism
Infectious diseases
Kluyveromyces - genetics
Kluyveromyces - growth & development
Kluyveromyces - physiology
Kluyveromyces bulgaricus
Medical sciences
Microbiology
Self-flocculation
Signal Transduction
Sodium Azide - pharmacology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
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Summary:Yeast flocculation appears to be dependent on several culture conditions such as nitrogen or carbon sources. In 0.2% glucose medium Kluyveromyces bulgaricus flocculation intensity is weak (10% at maximum) by comparison with flocculation in 2% glucose medium (85% maximum). Addition of glucose to K. bulgaricus in exponential growth phase in 0.2% glucose medium produced a rapid increase of the flocculation percentage during the 30 min following the addition of glucose. cAMP and 2,4-dinitrophenol showed similar effects while cAMP-dependent protein kinase (PKA) inhibitors exhibited an antagonist effect. Moreover, the induction of flocculation did not seem to imply translation of new proteins: cycloheximide had no effect, although growth was inhibited. The induction of flocculation mainly implies ATP hydrolysis for activation or secretion of galactose-specific receptors as demonstrated by treatment with NaN3. We propose a hypothesis that involves a PKA transduction signal leading to flocculation.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(01)00359-7