Measuring the effects of fractionated radiation therapy in a 3D prostate cancer model system using SERS nanosensors

Multicellular tumour spheroids (MTS) are three-dimensional cell cultures that possess their own microenvironments and provide a more meaningful model of tumour biology than monolayer cultures. As a result, MTS are becoming increasingly used as tumor models when measuring the efficiency of therapies....

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Bibliographic Details
Published in:Analyst (London) 2016-08, Vol.141 (17), p.556-561
Main Authors: Camus, Victoria L, Stewart, Grant, Nailon, William H, McLaren, Duncan B, Campbell, Colin J
Format: Article
Language:English
Subjects:
Cancer
Cell Culture Techniques
Cell Line, Tumor
Dose Fractionation, Radiation
Humans
Male
Monitors
Nanostructure
Prostate
Prostatic Neoplasms - radiotherapy
Raman spectroscopy
Spectrum Analysis, Raman
Spheroids, Cellular - radiation effects
Three dimensional models
Tumors
Viability
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Summary:Multicellular tumour spheroids (MTS) are three-dimensional cell cultures that possess their own microenvironments and provide a more meaningful model of tumour biology than monolayer cultures. As a result, MTS are becoming increasingly used as tumor models when measuring the efficiency of therapies. Monitoring the viability of live MTS is complicated by their 3D nature and conventional approaches such as fluorescence often require fixation and sectioning. In this paper we detail the use of Surface Enhanced Raman Spectroscopy (SERS) to measure the viability of MTS grown from prostate cancer (PC3) cells. Our results show that we can monitor loss of viability by measuring pH and redox potential in MTS and furthermore we demonstrate that SERS can be used to measure the effects of fractionation of a dose of radiotherapy in a way that has potential to inform treatment planning. Using Surface Enhanced Raman Spectroscopy to measure cell death caused by radiation in a 3D model of prostate cancer.
ISSN:0003-2654
1364-5528
DOI:10.1039/c6an01032f