Combination of competitive exclusion and immunisation with a live Salmonella vaccine in newly hatched chickens: Immunological and microbiological effects

• Published in: Research in veterinary science 2016-08, Vol.107, p.34-41
• Main Authors: Braukmann, M., Barrow, P.A., Berndt, A., Methner, U.
• Format: Article
• Language: English
• Subjects: Age; Animals; Attenuation; Birds; Cecum - microbiology; Chicken; Chickens; Chicks; Colonization; Combined vaccines; Competition; Competitive exclusion; Cytokines; Experiments; Exposure; Granulocytes; Hogs; Immune response; Immune system; Immunity; Immunology; Infections; Inhibition; Innate immunity; Intestine; Intestines - immunology; Juveniles; Leukocytes (granulocytic); Liver; Macrophages; Mucosa; Parameters; Phenols; Poultry; Poultry Diseases - prevention & control; Salmonella; Salmonella enteritidis; Salmonella enteritidis - immunology; Salmonella Infections, Animal - prevention & control; Salmonella live vaccine; Salmonella Vaccines - immunology; Transcription; Vaccination; Vaccines; Vaccines, Attenuated - immunology; Veterinary medicine; Virulence
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2016.05.001

In addition to evaluating the efficacy potential of a combined use of vaccination and competitive exclusion (CE) against Salmonella exposure in chicks at 3-days of age, a live Salmonella Enteritidis vaccine (SE-LV) and a CE culture were tested for their ability to induce parameters of the innate immunity. Whereas the invasive SE-LV induced an influx of granulocytes and macrophages as well as an increased transcription of several cytokines in the caecal mucosa, the CE culture did not demonstrate any differences in these parameters compared to controls. It is therefore highly probable that the effects observed with CE cultures are not due to the rapid stimulation of the immune system. The combined use of both preparations did not result in an additive intestinal exclusion effect of the challenge strain more pronounced than that after single administration of the CE culture. The combined use of the Salmonella live vaccine and the CE culture resulted in an additive protective effect and prevented completely the systemic dissemination of the Salmonella challenge strain. To exploit the potential of combined use of CE and vaccination further and most effectively, live Salmonella vaccines are needed that are despite their attenuation in virulence still capable to induce both intestinal colonisation- and invasion-inhibition effects against Salmonella exposure. •The efficacy potential of competitive exclusion and vaccination was studied.•We examined for the first time possible early immunological effects by a CE culture.•Colonisation- and invasion-inhibition effects by a live Salmonella vaccine were analysed.•The requirements for additive protective effects by both preparations are defined.