Transcription of a novel P450 gene varies with some factors (pollutant exposure, temperature, time, and body region) in a marine oligochaete (Thalassodrilides sp.)

• Published in: Marine pollution bulletin 2016-08, Vol.109 (1), p.344-349
• Main Authors: Ito, Mana, Ito, Katsutoshi, Ohta, Kohei, Hano, Takeshi, Onduka, Toshimitsu, Mochida, Kazuhiko
• Format: Article
• Language: English
• Subjects: Animals; Brackish; CYP4; cytochrome P-450; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; dose response; Environmental Monitoring; enzymes; exposure duration; gene expression; genes; Geologic Sediments; Marine; marine sediments; messenger RNA; metabolism; monitoring; mRNA expression; Oligochaeta; Oligochaeta - physiology; pollutants; Polycyclic aromatic hydrocarbon; polycyclic aromatic hydrocarbons; Polycyclic Aromatic Hydrocarbons - toxicity; polycyclic compounds; sediment contamination; Stress, Physiological; Temperature; Temperature response; transcription (genetics); Water Pollutants, Chemical - analysis; Water Pollutants, Chemical - toxicity; water pollution; Xenobiotics
• ISSN: 0025-326X; 1879-3363
• DOI: 10.1016/j.marpolbul.2016.05.055

Cytochrome P450 (CYP) enzymes play important roles in the metabolism of exogenous compounds such as polycyclic aromatic hydrocarbons (PAHs). A novel, full-length CYP gene (CYP4V30) was identified in the oligochaete Thalassodrilides sp. CYP4V30 mRNA expression was studied in worms exposed to PAH-polluted (Σ16PAHs; 37441ng/g dry weight) or unpolluted (Σ16PAHs; 19ng/g dry weight) sediment. CYP4V30 expression was much higher in worms exposed to contaminated sediments than in those exposed to unpolluted sediments at some temperatures (20 and 25°C) and exposure durations (11-fold increase at 20°C, 10-day exposure), but not at 15°C or other exposure durations (P