Bovine ovarian cells have (pro)renin receptors and prorenin induces resumption of meiosis in vitro

• Published in: Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2016-07, Vol.81, p.1-8
• Main Authors: Dau, Andressa Minussi Pereira, da Silva, Eduardo Pradebon, da Rosa, Paulo Roberto Antunes, Bastiani, Felipe Tusi, Gutierrez, Karina, Ilha, Gustavo Freitas, Comim, Fabio Vasconcellos, Gonçalves, Paulo Bayard Dias
• Format: Article
• Language: English
• Subjects: Aliskiren; Amides - pharmacology; Angiotensin II; Angiotensin II - metabolism; Animals; Cattle; Cells, Cultured; Colforsin - pharmacology; Corpus Luteum - drug effects; Corpus Luteum - physiology; Corpus Luteum - transplantation; Cumulus cell; Cumulus Cells - cytology; Cumulus Cells - drug effects; Cumulus Cells - physiology; Female; Fumarates - pharmacology; Granulosa Cells - cytology; Granulosa Cells - drug effects; Granulosa Cells - physiology; Humans; Meiosis - drug effects; Meiosis - physiology; Nucleotides, Cyclic - metabolism; Oocyte; Oocytes - cytology; Oocytes - drug effects; Oocytes - physiology; Ovarian Follicle - cytology; Ovarian Follicle - drug effects; Ovarian Follicle - physiology; Ovary - cytology; Ovary - drug effects; Ovary - physiology; Prorenin; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; Renin - antagonists & inhibitors; Renin - genetics; Renin - metabolism; Reproduction - drug effects; Reproduction - physiology; RNA, Messenger - genetics; RNA, Messenger - metabolism; Saralasin; Saralasin - pharmacology; Theca Cells - cytology; Theca Cells - drug effects; Theca Cells - physiology
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/j.peptides.2016.03.010

•The (pro)renin receptor ((P)RR) protein is present in all ovarian follicular cells.•(P)RR messenger ribonucleic acid is transcribed in oocytes and cumulus cells.•Prorenin induces the resumption of meiosis via an angiotensin II-independent pathway.•Prorenin induces the resumption of oocyte meiosis that has been blocked by forskolin. The discovery of a receptor that binds prorenin and renin in human endothelial and mesangial cells highlights the possible effect of renin-independent prorenin in the resumption of meiosis in oocytes that was postulated in the 1980s.This study aimed to identify the (pro)renin receptor in the ovary and to assess the effect of prorenin on meiotic resumption. The (pro)renin receptor protein was detected in bovine cumulus-oocyte complexes, theca cells, granulosa cells, and in the corpus luteum. Abundant (pro)renin receptor messenger ribonucleic acid (mRNA) was detected in the oocytes and cumulus cells, while prorenin mRNA was identified in the cumulus cells only. Prorenin at concentrations of 10−10, 10−9, and 10−8M incubated with oocytes co-cultured with follicular hemisections for 15h caused the resumption of oocyte meiosis. Aliskiren, which inhibits free renin and receptor-bound renin/prorenin, at concentrations of 10−7, 10−5, and 10−3M blocked this effect (P