Corticosterone Inhibits the Proliferation of C6 Glioma Cells via the Translocation of Unphosphorylated Glucocorticoid Receptor

Astroglial cells have been considered to have passive brain function by helping to maintain neurons. However, recent studies have revealed that the dysfunction of such passive functions may be associated with various neuropathological diseases, such as schizophrenia, Alzheimer’s disease, amyotrophic...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2016/07/01, Vol.39(7), pp.1121-1129
Main Authors: Nakatani, Yoshihiko, Amano, Taku, Takeda, Hiroshi
Format: Article
Language:English
Subjects:
Animals
C6 glioma cell
Cell Line, Tumor
Cell Proliferation - drug effects
Cell Survival - drug effects
corticosterone
Corticosterone - pharmacology
Glioma - metabolism
glucocorticoid
Hormone Antagonists - pharmacology
Membrane Potential, Mitochondrial - drug effects
Mifepristone - pharmacology
mineralocorticoid
phosphorylation
Rats
Receptors, Glucocorticoid - metabolism
Receptors, Mineralocorticoid - metabolism
Spironolactone - pharmacology
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Summary:Astroglial cells have been considered to have passive brain function by helping to maintain neurons. However, recent studies have revealed that the dysfunction of such passive functions may be associated with various neuropathological diseases, such as schizophrenia, Alzheimer’s disease, amyotrophic lateral sclerosis and major depression. Corticosterone (CORT), which is often referred to as the stress hormone, is a well-known regulator of peripheral immune responses and also shows anti-inflammatory properties in the brain. However, it is still obscure how CORT affects astroglial cell function. In this study, we investigated the effects of CORT on the proliferation and survival of astroglial cells using C6 glioma cells. Under treatment with CORT for 24h, the proliferation of C6 glioma cells decreased in a dose-dependent manner. Moreover, this inhibition was diminised by treatment with mifepristone, a glucocorticoid receptor (GR) antagonist, but not by spironolactone, a mineralocorticoid receptor (MR) antagonist, and was independent of GR phosphorylation and other GR-related intracellular signaling cascades. Furthermore, it was observed that the translocation of GR from the cytosol to the nucleus was promoted by the treatment with CORT. These results indicate that CORT decreases the proliferation of C6 glioma cells by modifying the transcription of a particular gene related to cell proliferation independent of GR phosphorylation.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.b16-00017