Dietary Oil Source and Selenium Supplementation Modulate Fads2 and Elovl5 Transcriptional Levels in Liver and Brain of Meagre (Argyrosomus regius)

• Published in: Lipids 2016-06, Vol.51 (6), p.729-741
• Main Authors: Silva-Brito, Francisca, Magnoni, Leonardo J., Fonseca, Sthelio Braga, Peixoto, Maria João, Castro, L. Filipe C., Cunha, Isabel, de Almeida Ozório, Rodrigo Otávio, Magalhães, Fernando Antunes, Gonçalves, José Fernando Magalhães
• Format: Article
• Language: English
• Subjects: Acetyltransferases - genetics; Animals; Aquaculture; Argyrosomus regius; Biomedical and Life Sciences; Biosynthesis; Brain - metabolism; Diet; Dietary Fats - administration & dosage; Dietary Supplements; Fatty acid desaturases; Fatty Acid Desaturases - genetics; Fatty acid elongase; Fatty Acids - metabolism; Fish; Fish oil replacement; Fish oils; Fish Proteins - genetics; Gene Expression Regulation - drug effects; Life Sciences; Lipid Metabolism - drug effects; Lipidology; Liver; Liver - metabolism; Meagre; Medical Biochemistry; Medicinal Chemistry; Microbial Genetics and Genomics; Neurochemistry; Nutrition; Original Article; Perciformes - genetics; Plant Oils - administration & dosage; Plant Oils - pharmacology; Polyunsaturated fatty acids; Selenium; Selenium - administration & dosage; Selenium - pharmacology; Vegetable oils
• ISSN: 0024-4201; 1558-9307
• DOI: 10.1007/s11745-016-4157-6

The meagre ( Argyrosomus regius ) is taking on increasing importance in the aquaculture industry. In view of the limited supply of fish oil (FO) as a feed ingredient, the study of the capacity to biosynthesize long-chain polyunsaturated fatty acids (LC-PUFA) from alternative dietary oil sources is important. We analyzed changes in fatty acid (FA) desaturase 2 ( fads2 ) and FA elongase 5 ( elovl5 ) mRNA levels in livers and brains in response to FO replacement with a blend of vegetable oils (VO) and selenium (Se) supplementation. Fish were fed for 60 days with either a diet containing FO or a diet including VO, each supplemented or not with organic Se. Results showed that fads2 and elovl5 transcription was higher in liver when fish were fed VO diets. The brain mRNA levels of both genes were not affected by the dietary replacement of FO by VO. FA composition in the liver and skeletal muscle was altered by FO replacement, particularly by decreasing eicosapentaenoic acid and docosahexaenoic acid contents. The α-linolenic, linoleic, and arachidonic acid contents increased in both liver and brain of fish fed VO diets. The effect of Se supplementation on lipid metabolism was evident only in fish fed FO, showing a decrease in the transcription of hepatic fads2 . Results indicate that the total replacement of FO by VO in diets modulates the expression of genes involved in LC-PUFA biosynthesis in meagre, affecting the FA profile of the fish flesh.