Does MW Radiation Affect Gene Expression, Apoptotic Level, and Cell Cycle Progression of Human SH-SY5Y Neuroblastoma Cells?

Neuroblastoma (NB) is a cancer that occurs in sympathetic nervous system arising from neuroblasts and nerve tissue of the adrenal gland, neck, chest, or spinal cord. It is an embryonal malignancy and affects infants and children. In this study, we investigated the effects of microwave (MW) radiation...

Full description

Saved in:
Bibliographic Details
Published in:Cell biochemistry and biophysics 2016-06, Vol.74 (2), p.99-107
Main Authors: Kayhan, Handan, Esmekaya, Meric Arda, Saglam, Atiye Seda Yar, Tuysuz, Mehmed Zahid, Canseven, Ayşe Gulnihal, Yagci, Abdullah Munci, Seyhan, Nesrin
Format: Article
Language:English
Subjects:
Apoptosis - radiation effects
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Biotechnology
Cell Biology
Cell Cycle - radiation effects
Cell Line, Tumor
Cell Survival - radiation effects
Gene Expression Regulation, Neoplastic - radiation effects
Humans
Infants
Iodides
Life Sciences
Microwaves
Nervous tissues
Neuroblastoma - pathology
Original Paper
Pharmacology/Toxicology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Neuroblastoma (NB) is a cancer that occurs in sympathetic nervous system arising from neuroblasts and nerve tissue of the adrenal gland, neck, chest, or spinal cord. It is an embryonal malignancy and affects infants and children. In this study, we investigated the effects of microwave (MW) radiation on apoptotic activity, cell viability, and cell cycle progression in human SH-SY5Y NB cells which can give information about MW radiation effects on neural cells covering the period from the embryonic stages to infants. SH-SY5Y NB cells were exposed to 2.1 GHz W-CDMA modulated MW radiation for 24 h at a specific absorption rate of 0.491 W/kg. Control samples were in the same conditions with MW-exposed samples but they were not exposed to MW radiation. The apoptotic activity of cells was measured by Annexin-V-FITC and propidium iodide staining. Moreover, mRNA levels of proliferative and cell cycle proteins were determined by real-time RT-PCR. The change in cell cycle progression was observed by using CycleTest-Plus DNA reagent. No significant change was observed in apoptotic activity of MW-exposed cells compared to control cells. The mRNA levels of c - myc and cyclin D1 were significantly reduced in MW group ( p  
ISSN:1085-9195
1559-0283
DOI:10.1007/s12013-016-0734-9