Single muscle fiber contractile properties in diabetic RAT muscle

ABSTRACT Introduction: Diabetes is associated with accelerated loss of muscle mass and function. We compared the contractile properties of single muscle fibers in young rat soleus muscle of uncontrolled streptozotocin‐induced diabetic animals (n = 10) and nondiabetic controls (n = 10). Methods: Sing...

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Bibliographic Details
Published in:Muscle & nerve 2016-06, Vol.53 (6), p.958-964
Main Authors: RodríGuez-Reyes, Natividad, RodríGuez-Zayas, Ana E., Javadov, Sabzali, Frontera, Walter R.
Format: Article
Language:English
Subjects:
Animals
atrophy
Blood Glucose - drug effects
Body Weight - drug effects
diabetes
Diabetes Mellitus, Experimental - pathology
Disease Models, Animal
Male
Muscle Contraction - physiology
Muscle Fibers, Skeletal - pathology
muscle power
Muscle, Skeletal - physiopathology
Myosin Heavy Chains - metabolism
myosin oxidation
Myosins - metabolism
Protein Carbonylation
Rats
Rats, Sprague-Dawley
specific force
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Summary:ABSTRACT Introduction: Diabetes is associated with accelerated loss of muscle mass and function. We compared the contractile properties of single muscle fibers in young rat soleus muscle of uncontrolled streptozotocin‐induced diabetic animals (n = 10) and nondiabetic controls (n = 10). Methods: Single fiber maximal force, shortening velocity, and power were assessed during maximal activation with calcium using the slack test 4 weeks after induction. Myosin heavy chain expression was determined using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Oxidized myosin levels were detected by analyzing protein carbonyls in muscle homogenates. All fibers expressed the type I myosin heavy chain isoform. Results: Diabetic rats had higher blood glucose (537 vs. 175 mg/dl; P 
ISSN:0148-639X
1097-4598
DOI:10.1002/mus.24988