Immunostimulatory CpG oligodeoxynucleotides stimulate expression of IL-1β and interferon-like cytokines in rainbow trout macrophages via a chloroquine-sensitive mechanism

Synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs are known to stimulate immune responses and are potent adjuvants in higher vertebrates, but so far the effects in fish are poorly described. We here report that CpG ODNs induce IL-1β expression and production of interferon-lik...

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Bibliographic Details
Published in:Fish & shellfish immunology 2001-11, Vol.11 (8), p.673-682
Main Authors: Jørgensen, J.B., Zou, J., Johansen, A., Secombes, C.J.
Format: Article
Language:English
Subjects:
Brackish
Freshwater
head kidney
interleukin 1 beta
Marine
oligodeoxynucleotides
Oncorhynchus mykiss
rainbow trout, CpG motif, cytokine, interferon, IL-1β, macrophages
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Summary:Synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs are known to stimulate immune responses and are potent adjuvants in higher vertebrates, but so far the effects in fish are poorly described. We here report that CpG ODNs induce IL-1β expression and production of interferon-like cytokines in rainbow trout head-kidney macrophages, whereas ODNs with an inverted motif (GpC) have a much less stimulatory effect. We further demonstrate that endosomal maturation is essential for CpG signalling, as chloroquine, a compound known to block endosomal acidification, inhibits cytokine expression in the macrophages.
ISSN:1050-4648
1095-9947
DOI:10.1006/fsim.2001.0344