Enhanced mannan-derived fermentable sugars of palm kernel cake by mannanase-catalyzed hydrolysis for production of biobutanol

• Published in: Bioresource technology 2016-10, Vol.218, p.257-264
• Main Authors: Shukor, Hafiza, Abdeshahian, Peyman, Al-Shorgani, Najeeb Kaid Nasser, Hamid, Aidil Abdul, Rahman, Norliza A., Kalil, Mohd Sahaid
• Format: Article
• Language: English
• Subjects: Acetone - chemistry; Analysis of Variance; Arecaceae - chemistry; beta-mannosidase; beta-Mannosidase - chemistry; biobutanol; Biobutanol production; Biofuels; Bioreactors; Butanols - chemistry; Carbohydrates - chemistry; Catalysis; Clostridium - metabolism; Clostridium saccharoperbutylacetonicum; depolymerization; Enzymatic hydrolysis; Ethanol - chemistry; Fermentation; glucose; Glucose - chemistry; hydrolysates; Hydrolysis; Mannan-derived monomeric sugar; Mannanase; Mannans - chemistry; mannose; Mannose - chemistry; Palm kernel cake; temperature
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2016.06.084

[Display omitted] •Palm kernel cake (PKC) was hydrolyzed by mannanase.•The effect of operating factors on the enzymatic hydrolysis of PKC was studied.•Mannose was the main fermentable sugar released with glucose to a lower extent.•The total sugar of 71.54±2.54g/L was produced in optimum conditions.•Maximum biobutanol production of 3.27±1.003g/L was obtained. Catalytic depolymerization of mannan composition of palm kernel cake (PKC) by mannanase was optimized to enhance the release of mannan-derived monomeric sugars for further application in acetone-butanol-ethanol (ABE) fermentation. Efficiency of enzymatic hydrolysis of PKC was studied by evaluating effects of PKC concentration, mannanase loading, hydrolysis pH value, reaction temperature and hydrolysis time on production of fermentable sugars using one-way analysis of variance (ANOVA). The ANOVA results revealed that all factors studied had highly significant effects on total sugar liberated (P