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Nanomechanical sandwich assay for multiple cancer biomarkers in breast cancer cell-derived exosomes
The use of exosomes as cancer diagnostic biomarkers is technically limited by their size, heterogeneity and the need for extensive purification and labelling. We report the use of cantilever arrays for simultaneous detection of multiple exosomal surface-antigens with high sensitivity and selectivity...
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| Published in: | Nanoscale 2016-08, Vol.8 (33), p.15137-15141 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c342t-32b466ef958bcf60ff5e8582a61fa784bb6eb32233007945a51f039917de7aba3 |
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| cites | cdi_FETCH-LOGICAL-c342t-32b466ef958bcf60ff5e8582a61fa784bb6eb32233007945a51f039917de7aba3 |
| container_end_page | 15141 |
| container_issue | 33 |
| container_start_page | 15137 |
| container_title | Nanoscale |
| container_volume | 8 |
| creator | Etayash, H McGee, A. R Kaur, K Thundat, T |
| description | The use of exosomes as cancer diagnostic biomarkers is technically limited by their size, heterogeneity and the need for extensive purification and labelling. We report the use of cantilever arrays for simultaneous detection of multiple exosomal surface-antigens with high sensitivity and selectivity. Exosomes from breast cancer were selectively identified by detecting over-expressed membrane-proteins CD24, CD63, and EGFR. Excellent selectivity however, was achieved when targeting the cell-surface proteoglycan, Glypican-1 at extraordinary limits (200 exosomes per mL, 0.1 pg mL
1
).
The use of exosomes as cancer diagnostic biomarkers is technically limited by their size, heterogeneity and the need for extensive purification and labelling. |
| doi_str_mv | 10.1039/c6nr03478k |
| format | article |
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1
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1
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| fulltext | fulltext |
| identifier | ISSN: 2040-3364 |
| ispartof | Nanoscale, 2016-08, Vol.8 (33), p.15137-15141 |
| issn | 2040-3364 2040-3372 |
| language | eng |
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| source | Royal Society of Chemistry:Jisc Collections:Royal Society of Chemistry Read and Publish 2022-2024 (reading list) |
| subjects | Arrays Biomarkers Biomarkers, Tumor - analysis Breast Breast Neoplasms - metabolism Cancer CD24 Antigen - metabolism Cell Line, Tumor ErbB Receptors - metabolism Exosomes - chemistry Heterogeneity Humans Labelling Nanoparticles Nanostructure Selectivity Tetraspanin 30 - metabolism |
| title | Nanomechanical sandwich assay for multiple cancer biomarkers in breast cancer cell-derived exosomes |
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