Impact of acquisition of 16S rRNA methylase RmtB on the fitness of Escherichia coli

• Published in: Journal of global antimicrobial resistance. 2016-09, Vol.6, p.32-38
• Main Authors: Ou, Bingming, Chen, Lin, Song, Yujie, Yang, Ying, Zhang, Qian, Yang, Yi, Li, Luan, Tham, Wai Liang, Francis, David H., Zhu, Guoqiang
• Format: Article
• Language: English
• Subjects: 16S rRNA methylase RmtB; Aminoglycosides; Biofilm formation; Competitive ability; Deletion mutation; Growth rate
• ISSN: 2213-7165; 2213-7173
• DOI: 10.1016/j.jgar.2016.02.004

•Acquisition of the rmtB gene in Escherichia coli entails a considerable fitness cost to the bacteria.•E. coli carrying RmtB has a slightly reduced growth rate and a decreased competitive capacity.•16S rRNA methylase RmtB does not affect biofilm formation or motility of bacteria. The aim of this study was to elucidate the biological phenotypes of 16S rRNA methylase RmtB in Escherichia coli and the impact of RmtB acquisition on the fitness of the target bacterium. An rmtB in-frame deletion mutant in E. coli was constructed using a suicide vector (pDMS197)-based double crossover allelic exchange, and its corresponding complemented strain was established. Combined studies of microdilution susceptibility testing, conjugation experiments, growth kinetics assays, competitive experiments, biofilm formation tests and motility assays were performed to study the rmtB-mediated fitness among the prototype E. coli strain, its isogenic mutant and the corresponding complemented strain. The minimum inhibitory concentrations (MICs) of 4,6-disubstituted 2-deoxystreptamines for the rmtB wild-type strain, its isogenic mutant and the complemented strain were ≥1024, ≤2 and ≥1024mg/L, respectively. Both the growth rates and the competitive abilities of the wild-type and complemented strains were relatively inferior to the ΔrmtB mutant. There was no significant difference in biofilm formation and motility among the three strains. In conclusion, the data presented here suggest that acquisition of the 16S rRNA methylase gene rmtB in E. coli can exact a fitness cost on the bacteria, subsequently reducing the growth rate slightly and decreasing the competitive capacity of the bacterium, whereas it does not affect biofilm formation or motility.