Structural characteristics of oxalate-soluble polysaccharides of Sosnowsky's hogweed (Heracleum sosnowskyi Manden)

•The chemical structure of pectin and AGP of H. sosnowskyi has been characterized.•The side chains of the hogweed pectin are composed of AG-I and 1,5-α-L-arabinan.•AGP consists of 1,3-, 1,6-, 1,3,6-β-Galp, 1,5-, 1,3,5-a-Araf, 4-O-Me-, 1,4-β-GlcpA.•A part of pectin is probably covalently linked to AG...

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Bibliographic Details
Published in:Carbohydrate polymers 2016-11, Vol.153, p.66-77
Main Authors: Makarova, Elena N., Shakhmatov, Evgeny G., Belyy, Vladimir A.
Format: Article
Language:English
Subjects:
Arabinogalactan II
Arabinogalactan proteins
Galactans - analysis
Heracleum - chemistry
Heracleum sosnowskyi
Magnetic Resonance Spectroscopy
Mucoproteins - analysis
NMR
Oxalates - chemistry
Pectin
Pectins - analysis
Plant Proteins - analysis
Polysaccharides - analysis
Solubility
Structure
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Summary:•The chemical structure of pectin and AGP of H. sosnowskyi has been characterized.•The side chains of the hogweed pectin are composed of AG-I and 1,5-α-L-arabinan.•AGP consists of 1,3-, 1,6-, 1,3,6-β-Galp, 1,5-, 1,3,5-a-Araf, 4-O-Me-, 1,4-β-GlcpA.•A part of pectin is probably covalently linked to AGP.•AGP is directly linked to the RG-I region of the pectin. Arabinogalactan proteins (AGP) and pectic polysaccharides were isolated from above-ground parts of Heracleum sosnowskyi. The structural study has shown that a linear region of the pectic macromolecules consists of 1,4-α-d-galactopyranosyluronan blocks partially methyl esterified and acetylated. The branched region consists of 3-O- and partially 2-O-acetylated rhamnogalacturonan I. Side chains of the RG-I backbone include the regions of arabinogalactan I and branched 1,5-α-l-arabinan. The carbohydrate part of AGP consists of arabinogalactan II with a 1,3-β-d-Galp main chain. The side chains of the branched area of AG-II are composed of 1,6-β-d-Galp, 1,5-, 1,3,5-α-l-Araf, 4-O-Me-β-d-GlcA and 1,4-β-d-GlcpA, and non-reducing ends residues of β-d-Galp, α-l-Araf, α-l-Rhap and α-l-Fucp. The branch points of the main and side chains are formed by 3,6-di-O-substituted β-d-Galp. It was found that at least a portion of pectin is probably covalently linked to AGP, wherein AGP is linked to RG-I, but not with galacturonan.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2016.07.089