Structural characterization of allomelanin from black oat

• Published in: Phytochemistry (Oxford) 2016-10, Vol.130, p.313-320
• Main Authors: Varga, Mónika, Berkesi, Ottó, Darula, Zsuzsanna, May, Nóra Veronika, Palágyi, András
• Format: Article
• Language: English
• Subjects: Avena - chemistry; Avena sativa (Poaceae); Coumaric Acids - chemistry; FT-IR spectroscopy; MALDI-TOF MS; Melanin; Melanins - analysis; Molecular Weight; Oat; p-Coumaric acid; Pigmentation; Propionates; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectroscopy, Fourier Transform Infrared
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/j.phytochem.2016.07.002

The brown to black coloration found in plants is due to the melanins, which have been relatively poorly investigated among the plant pigments. The aim of this work was to study the dark pigment extracted from the black oat hull with respect to composition and structure. Ultraviolet–visible (UV–Vis) spectroscopy, electron paramagnetic resonance (EPR) spectroscopy, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and Fourier transform infrared (FT-IR) spectroscopy were applied for the characterization of the pigment. UV–Vis spectroscopy revealed that the extracted material displays a broadband, structureless absorption profile a common feature of melanins. MALDI-TOF MS measurements demonstrated that oat melanin is a homopolymer built up from p-coumaric acid and consists mainly of low molecular weight (527–1499 Da) oligomers of 3–9 monomer units. The tetramer oligomer proved to be dominant. The results of the FT-IR analysis indicated that oat melanin is a fully conjugated aromatic system containing tetrasubstituted aromatic rings linked by CC coupling. The in vitro preparation of melanin from p-coumaric acid by horseradish peroxidase was performed for comparison. The resulting polymer consisted of oligomers of 4–9 monomer units similarly to those in oat melanin. However, the building blocks proved to be connected to each other via COC linkages in contrast with the CC linkages in oat melanin. [Display omitted] •The pigment extracted from the black oat was identified as melanin.•Structure was determined by MALDI-TOF MS and FT-IR spectroscopy.•In vitro preparation of oat melanin was attempted.•Structure of the in vitro prepared polymer was elucidated.