Comparison of different extraction methods and HPLC quantification of prenylated and unprenylated phenylpropanoids in raw Italian propolis

[Display omitted] •4′-Geranyloxyferulic and boropinic acids, auraptene and 7-isopentenyloxycoumarins in propolis.•An HPLC-UV/Vis method was successfully applied for their quali-quantitative analysis.•New insights on the chemical composition of propolis are provided. In this paper the presence of sel...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2016-09, Vol.129, p.219-223
Main Authors: Taddeo, Vito Alessandro, Epifano, Francesco, Fiorito, Serena, Genovese, Salvatore
Format: Article
Language:English
Subjects:
4′-Geranyloxyferulic acid
Auraptene
beta-Cyclodextrins - chemistry
Boropinic acid
Chromatography, High Pressure Liquid - methods
Coumaric Acids - chemistry
Coumarins - chemistry
Ferulic acid
Italy
Propanols - chemistry
Propolis
Propolis - chemistry
Umbelliferone
Umbelliferones - chemistry
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Summary:[Display omitted] •4′-Geranyloxyferulic and boropinic acids, auraptene and 7-isopentenyloxycoumarins in propolis.•An HPLC-UV/Vis method was successfully applied for their quali-quantitative analysis.•New insights on the chemical composition of propolis are provided. In this paper the presence of selected prenylated and unprenylated phenylpropanoids, namely ferulic acid 1, boropinic acid 2, 4′-geranyloxyferulic acid 3, umbelliferone 4, 7-isopentenyloxycoumarin 5, and auraptene 6, have been determined in Italian raw propolis after having been extracted with different methodologies. An aqueous solution of β-cyclodextrin was the best extraction method for ferulic acid 1, treatment with indifferently EtOH or aqueous β-cyclodextrin were the most effective one for umbelliferone 4, boropinic acid 2 gave the best yields either with H2O/β-cyclodextrin or olive oil treatment or in biphasic systems, maceration with biphasic mixtures of aqueous β-cyclodextrin and olive oil was seen to be the most effective procedure for 7-isopentenyloxycoumarin 5, the only method providing significant quantities of 4′-geranyloxyferulic acid 3 was the maceration of raw propolis with olive oil, and finally auraptene 4 was best extracted with absolute EtOH. “Classic” maceration in general performed better than ultrasound-assisted one.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2016.07.006