Long-term repeated biodesulfurization by immobilized Rhodococcus erythropolis KA2-5-1 cells

In this study, biodesulfurization (BDS) was carried out using immobilized Rhodococcus erythropolis KA2-5-1 in n-tetradecane containing dibenzothiophene (DBT) as a model oil (n-tetradecane/immobilized cell biphasic system). The cells were immobilized by entrapping them with calcium alginate, agar, ph...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2001-04, Vol.55 (3), p.374-378
Main Authors: NAITO, Manabu, KAWAMOTO, Takuo, FUJINO, Kazuhito, KOBAYASHI, Morio, MARUHASHI, Kenji, TANAKA, Atsuo
Format: Article
Language:English
Subjects:
Agar
Alginates
Alkanes - metabolism
Biodegradation, Environmental
Biological and medical sciences
Biotechnology
calcium alginate
Cells, Immobilized - metabolism
desulfurization
dibenzothiophene
Energy
Fundamental and applied biological sciences. Psychology
Glucuronic Acid
Hexuronic Acids
Industrial applications and implications. Economical aspects
Polymers
Rhodococcus - metabolism
Rhodococcus erythropolis
sulfur
Sulfur Compounds - metabolism
tetradecane
Thiophenes - metabolism
Time Factors
Use of microorganisms or biopolymers in fossil fuel recovery
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Summary:In this study, biodesulfurization (BDS) was carried out using immobilized Rhodococcus erythropolis KA2-5-1 in n-tetradecane containing dibenzothiophene (DBT) as a model oil (n-tetradecane/immobilized cell biphasic system). The cells were immobilized by entrapping them with calcium alginate, agar, photo-crosslinkable resin prepolymers (ENT-4000 and ENTP-4000), and urethane prepolymers (PU-3 and PU-6); and it was found that ENT-4000-immobilized cells had the highest DBT desulfurization activity in the model oil system without leakage of cells from the support. Furthermore, ENT4000-immobilized cells could catalyze BDS repeatedly in this system for more than 900 h with reactivation; and recovery of both the biocatalyst and the desulfurized model oil was easy. This study would give a solution to the problems in BDS, such as the troublesome process of recovering desulfurized oil and the short life of BDS biocatalysts.
ISSN:0175-7598
1432-0614
DOI:10.1007/s002530000527